中文摘要：

目的探讨尖锐湿疣患者外周血T细胞亚群及NK细胞水平及其与尖锐湿疣临床复发的关系。方法用免疫荧光法检测杭州市第一人民医院就诊的94例尖锐湿疣病例和50例正常对照组外周血中T细胞亚群及NK细胞水平，CO2激光清除病例疣体后每2周复查1次，随访持续12周，并统计复发率和复发次数，并比较复发和未复发病例、不同型别HPV患者外周血T细胞亚群水平。结果病例总体复发率为65．96％（62／94），平均复发次数为2．23次。尖锐湿疣病例外周血CD4＋T细胞占（32．41±8．14）％，低于对照组（t=4．52，P〈0．01）；CD8＋T细胞占（31．56±7．95）％，高于对照组（t=5．44，P〈0．01）；NK细胞占（19．86±6．29）％，低于对照组（t=2．12，P〈0．05）。与尖锐湿疣未复发病例相比，复发病例外周血CD8＋T细胞较高，占（33．64±7．71）％；而CD4／CD8比值较低，为（0．97±0131），差异均有统计学意义（t=3．79、4．34，P均〈0．01）。与HPV16／18单一型别感染和HPV6／11／16／18型合并感染病例相比，HPV6／11单一型别感染病例外周血CD8＋T细胞水平较低、CD4＋／CD8＋比值较高（q=3．37～3．45，P均〈0．05）。结论尖锐湿疣患者存在细胞免疫缺陷，而高危型HPV感染病例有更高的免疫缺陷风险。CD8＋T细胞水平和CD4＋／CD8＋比值有助于判断尖锐湿疣预后。

英文摘要：

Objective To evaluate the variation of peripheral blood T lymphocyte subsets and NK cells of patients with condyloma acuminatum （CA）, and the relationship with the clinical recurrence rate. Methods Peripheral blood T lymphocyte subsets and NK cells of 94 CA cases and 50 normal controls of patients from Hangzhou First People＇s Hospital were detected by immunofluorescence method. The cases whose warts were cleared by using CO2 laser, observed the therapeutic effect per two weeks. The rate and the times of recurrence were examined after 12-week follow-up. The peripheral blood T lymphocyte subsets levels of different patients and types of HPV were compared. Results After 12-week follow-up, the rate and the average times of recurrence were 65.96%（62/94）and 2.23 in all of the CA cases. The CD4＋T cell percentage in CA cases was （32.41±8.14）%, lower than that in control group （t=4.52, P〈0.01）; the CD8＋T cell accounted for （31.56±7.95）%, higher than control group （t=5.44, P〈0.01）; NK cells accounted for （19.86±6.29）%, also lower than control group （t=2.12, P〈0.05）. Compared with the CA cases without recurrence, the CD8＋ T cell in recurrence ones were higher, accounted for （33.64±7.71）%（t=3.79, P〈0.01）; the CD4＋/CD8＋ cell ratio was lower （0.97±0.31, t=4.34, P〈0.01）. The level of CD8＋T cell percentage in the cases infected with sole HPV6/11 genotype, was lower than HPV6/11/16/18 genotype coinfeeted cases, or sole HPV16/18 genotype infected ones（q=3.45 and 3.37,P〈0.05）. The CD4＋/CD8＋ cell ratio in the sole HPV6/11 genotype infected ones was higher than HPV6/11/16/18 genotype eoinfected or sole HPV16/18 genotype infected ones （q=3.38 and 3.38, P〈0.05）. Conclusions The cell-mediated immunity exists in the patients with CA, and the high-risk HPV genotype infection patients have higher probability of cell-mediated immunity. The level of CD8＋T cell percentage and CD4＋/CD8＋ cell ratio can be the helpful molecular biomarkers in risk asses