中文摘要：

目的检测脑缺血再灌注后不同时间点轴突生长抑制因子Nogo-A在损伤白质区的基因表达，探讨Nogo—A与缺血再灌注白质损伤的可能关系。方法制备大鼠大脑中动脉缺血再灌注（MCAO）模型，实时定量聚合酶链反应方法（Relative Quantification PCR，RQ—PCR）检测各时间点Nogo—A在损伤白质区域的基因表达。结果在损伤白质区域，Nogo—A基因表达在缺血再灌注1d时表达量开始减少（与假手术组相比，P〈0．05），7d时降至最低，14～21d表达量开始上调，21d时仍低于假手术组水平（与假手术组相比，P〈0．001）。结论缺血再灌注损伤后白质区域的Nogo—A基因表达呈现动态变化规律，针对Nogo—A进行干预有望为缺血再灌注损伤后的脑保护治疗找到新的靶点。

英文摘要：

Objective To study the expression of Nogo-A in cerebral white matter after focal cerebral ischemia-reperfusion（I/R） in rats, and explore the relationship between Nogo-A and cerebral white matter lesions after ischemia. Methods Sprague-Dawley male rats were subjected to 2 h of middle cerebral artery occlusion （MCAO）. Relative quantification PCR（RQ-PCR） was performed to examine the expressions of Nogo-A gene at different reperfusion time. Results The expression level of Nogo-A gene in the white matter was decreased in 1 d group（P 〈0.05 vs sham group） ,and was the weakest in 7 d group. It was increased in 14 -21 d group, and in 21 d the expression level was still lower than that of sham group （ P 〈 0. 001 ）. Conclusions Nogo-A expreesion has dynamic changes in the white matter after focal cerebral I/R. Nogo-A could be as one of the targets for neuroprotective therapy after cerebral ischemia.