中文摘要：

目的探讨γ过氧化物酶体增殖物激活受体（peroxisome proliferator activated receptor-7，PPAR-γ）的配体15-脱氧-△^12,14-前列腺素J2（15-deoxy-△^12,14-prostagliandxinJ2，15d—PGJ2）对兔耳增生性瘢痕I型胶原、结缔组织生长因子（connective tissue growth factor，CTGF）、α平滑肌肌动蛋白（a—smooth muscle actin，α—SMA）表达的影响，探讨15d—PGJ2防治增生性瘢痕的可能性。方法选取新西兰大白兔18只，在兔耳腹侧面制作2cm×3cm全层皮肤缺损创面，每耳2个，共计72个，建立兔耳增生性瘢痕动物模型，随机分为2组，一组为实验组，另一组为对照组，分别用15d—PGJ2和生理盐水行瘢痕内注射，1次／d，共7次。停药后第7、14、21天两组同时取材；每组每次切取12个组织块。应用免疫组织化学检测I型胶原、CTGF和α—SMA的表达。结果各组兔耳腹侧创面愈合后均不同程度出现类似人增生性瘢痕的组织块。与对照组相比，15dPGJ2注射后瘢痕体积缩小，变软变平，色泽轻度变浅。在各个时间点15d—PGJ2组I型胶原、CTGF和α-SMA的表达均较对照组低，且差异有统计学意义（P〈0．05）。结论PPAR-γ的配体15d—PGJ2可降低瘢痕内I型胶原、CTGF和α—SMA的含量，引起瘢痕萎缩，有一定防治瘢痕的作用，可能为临床治疗增生性瘢痕提供一条新的途径。

英文摘要：

Objective To investigate the effect of 15d-PGJ2 on the expression of collagen type, CTGF and α-SMA in the hypertrophic scar in the rabbit ear, and the possibility of hypertrophic scar treated by 15d-PGJ2. Methods 18 New Zealand white rabbits were used to establish a hypertrophic scar model on the rabbit ear. The wounds were established as follows： 2 cm ;〈 3 cm wounds with total skin loses on the ventral side, 2 wounds for each ear, totally 72 wounds. The wounds were randomly divided into the 15d-PGJ2 treatment group and NS control group. 20μl 15d-PGJ2 or NS was injected into the ear scar once a day for 7 days. At 7, 14 and 21 days after the injection, 12 scars of each group were harvested. The expression of collagen type I , CTGF and α-SMA was detected by immunohistochemical method. Results Excessive dermal scars on rabbit ears that were similar to human hypertrophic scar appeared in the two groups. Compared with the NS-treated scars group, the 15d-PGJ2-treated scars appeared to be smaller, softer, flatter and lighter in color. The expression of collagen type I , CTGF and α-SMA in the 15d-PGJ2 group was significantly decreased as compared with that in the control group at different time points（P〈0.05）. Conclusion 15d-PGJ2, the ligand of PPAR 7, can reduce the expression of collagen type I ,CTGF and α-SMA of hypertrophic scar in the rabbit ears and plays an important role in the prevention and treatment of hypertrophic scar. It may provide a new approach for the treatment of hypertrophic scar in clinical setting.