中文摘要：

检测紫外光（ultra violet，UV）照射后棉铃虫HMG-CoA还原酶基因、卵黄原蛋白基因的表达量及棉铃虫产卵量的变化。提取经UV照射后棉铃虫雌虫的总RNA并反转录为cDNA。利用荧光定量PCR（qPCR）技术检测HMG-CoA还原酶基因及卵黄原蛋白基因的表达量。UV照射后，统计棉铃虫雌虫的产卵量。结果表明：UV照射0.5~5.0 h，棉铃虫HMG-CoA还原酶基因的表达量显著增加； UV照射1.0~5.0 h， 24 h后取样发现棉铃虫卵黄原蛋白基因的表达量显著增加；UV照射1.0~4.0 h，棉铃虫雌虫的产卵量显著增加。研究结果证实UV照射能影响棉铃虫hmgr、vg的表达量和雌虫的产卵量。

英文摘要：

The objective of this study is to investigate the relative expression of HMG-CoA reduc- tase （hmgr） and vitellogenin （vg） mRNA of Helicoverpa armigera adults under UV light irradiation, together with the fecundity variation of UV light irradiation group. Quantitative real-time PCR （qPCR） technique was applied to detect the expression of hmgr and vg of H. armigera of the UV light irradia- tion group. Total RNA was extracted from the whole body of H. armigera, and synthesized to cDNA via reverse transcription. Specific primers on hmgr, vg and β-actin in GenBank were used to carry out qPCR. In addition,this study also recorded the number of the eggs for female adults after UV light irra- diation. The expression of hmgr of H. armigera significantly increased after the exposure to UV light irradiation from 0. 5 h to 5.0 h, which was higher than that of control after UV light irradiation, while the vg expression increased 24 h later after UV light irradiation. The results also suggest that the fecun- dity of female adults significantly increased after UV treatment of 1.0 h to 4. 0 h. This study is the first analysis of differentially expressed mRNAs in phototactic insects under UV light irradiation conditions and gives new insights into the adaptation mechanisms responsive to UV light irradiation stress.