在显微镜下观察柑橘大实蝇Bactrocera minax(Enderlein)雌成虫自羽化后不同日龄的卵巢形态特征,测量并统计卵巢长度、卵巢宽度、卵巢指数、卵泡长度和卵巢成熟卵量等指标,根据卵巢形态特征和卵巢发育程度进行等级划分。结果表明,柑橘大实蝇雌虫卵巢发育进度可分为5个等级:雌虫羽化后1~10d卵巢发育为Ⅰ级(卵巢发育初期);10~18d为Ⅱ级(卵黄沉积期);18~23d为Ⅲ级(成熟待产期);23~33d为Ⅳ级(产卵盛期);羽化33d后为Ⅴ级(产卵末期)。
The Chinese citrus fly,Bactrocera minax(Enderlein),is one of the most harmful pests for citrus plants,which caused serious damage in recent years in China.In this study,we observed the ovarian development of B.minaxunder the microscope by measuring ovary length and width,ovarian index,follicle length,and the number of mature eggs.The results indicated that ovarian development of B.minaxcould be divided into five stages as follows:previtellogenic stage(Ⅰ),vitellogenic deposition stage(Ⅱ),expectant stage of mature eggs(Ⅲ),peak stage of oviposition(Ⅳ),last stage of oviposition(Ⅴ),which lasted for 1-10 days,10-18 days,18-23 days,23-33 days and 33 days after adult eclosion,respectively.The results could serve as a grading criterion for assessing the sexual maturation in the Chinese citrus fly,and also benefit for the monitoring and control of B.minaxin the field.