中文摘要：

目的通过比较NOD1受体在脓毒症大鼠和正常大鼠肾组织中的表达强度，探讨NODl受体在脓毒症诱导肾损伤中的作用。方法48只Wistar大鼠随机分为四组：假手术组（SHAM组），腹腔LPS注射组（LPS组），腹主动脉阻断组（AAC组），腹主动脉阻断＋腹腔LPS注射组（AAC＋LPS组）。术后8h处死大鼠取血清和肾组织，测定血肌酐（Cr）和尿素氮（BUN）水平。HE染色观察肾脏组织病理损伤，免疫组化检测肾组织NODl受体蛋白的表达和分布，frr—PCR检测肾组织NODl受体mRNA的表达。结果四组大鼠中，AAC＋LPS组大鼠死亡率最高；与SHAM组比较，LPS组、AAC组和AAC＋LPS组的血清Cr和BUN明显升高（P〈0．05），但LPS组和AAC组差异无统计学意义（P〉0．05）；免疫组化和RT—PCR显示，NOD1受体蛋白和mRNA的表达强度在SHAM组、LPS组、AAC组和AAC＋LPS组依次递增（P〈0．05），而LPS组和AAC组差异无统计学意义（P〉0．05）。结论NOD1受体在严重脓毒症大鼠的肾脏损伤中发挥了重要的作用。

英文摘要：

Objective To explore the expression of NOD1 receptor in the kidney of severe sepsis rats induced by abdominal aorta combined with intraperitoneal injection of lipopolysaccharide. Methods Forty - eight Wistar rats were divided into four groups randomly： SHAM group, LPS group, AAC group, and AAC ＋ LPS group. Rats were sacrificed at 8 h after operation, serum and kidney tissue were collected for detecting level of the blood creatinine （Cr） and urea nitrogen （BUN） level in serum. The renal tissue injury was observed by HE staining, immunohistochemical stainning was used to detect the NOD1 receptor protein expression and distribution in kidney, PT - PCR was used to detect the NOD1 receptor mRNA expression. Results The mortality rate in the AAC ＋ LPS group is highest than other groups. Compared with the SHAM group, serum Cr, BUN and NODI mRNA and protein expression in kidney were significantly increased （P 〈 0.05 ） in the LPS group, AAC group and AAC ＋ LPS group, those index in AAC group were slightly higher then in LPS groups, however there had no significant difference between LPS group and AAC group （P 〉 0.05 ）. Conclusion NOD1 receptor may play an important role in the progress of acute kidney injury induced by severe sepsis.