中文摘要：

目的研究Nogo—A基因沉默对PC12细胞多巴胺释放的影响。方法构建靶向Nogo—A的短发夹样RNA（shRNA）真核表达载体，并经脂质体转染PC12细胞，以逆转录聚合酶链反应（RT-PCR）和免疫印迹法检测对Nogo—AmRNA及蛋白的抑制效应。采用高效液相色谱仪（HPLC）检测转染前后多巴胺释放的变化。结果将重组质粒转染到PCI2细胞48h后，Nogo—A基因表达明显受抑、多巴胺释放量减少。结论Nogo-A可能参与PC12细胞多巴胺的释放。

英文摘要：

Objective To study the effect of Nogo-A gene silence on dopamine release in PC12 cells. Methods The small hairpin RNA（shRNA） eukaryotic expression vector targeting Nogo-A gene was constructed and transfected into cultured PCI2 cells by lipofecamine2000. The inhibiting effect was detected by semi-quantitative reverse transcription（PCR） and Western blotting analysis. The dopamine release was detected by HPLC （high performance liquid chromatography） after transfection. Results The Nogo-A gene expression was inhibited and the dopamine release decresed significantly 48 hours after transfection. Conclusion The reasults suggest that Nogo-A be involved in the mechanism of dopamine realese in PC12 cells.