中文摘要：

采用200 mg·kg^-1·d^-1的D-半乳糖诱导小鼠45 d建立糖基化模型,通过新物体识别检测模型组与正常对照组小鼠行为学差异,进一步利用酶联免疫法检测小鼠脑、心、肝、脾、肺、肾、皮肤、肌肉和血清中的糖基化末端产物和相关受体的表达量（AGEs、RAGE、sRAGE和esRAGE）,分析该模型中4种指标的变化情况。结果表明模型组小鼠的辨别指数显著低于空白组（P〈0.01）,模型组小鼠各组织器官中的糖基化末端产物和相关受体的表达量显著高于空白组（P〈0.01）;D-半乳糖慢性处理45 d后的小鼠从行为上有一定的迟缓,同时在对各个不同组织器官的生理指标的检测中发现机体处于高糖基化状态,可用于研究AGEs及其受体的发病机理和药物治疗作用途径,并且可以选用多个组织器官作为实验靶点。旨在为颈背部皮下注射D-半乳糖致糖基化小鼠模型提供理论依据。

英文摘要：

To provide a theoretical basis of a new rat model of glycation,the mice of glycation were induced by injecting D-galactose（ 200 mg kg^-1 d^-1） using injecting method for 45 days,which were measured the behavioral differences from model group to others and detected the index related to the glycation as AGEs,RAGE,sRAGE and esRAGE in variety organs of mice（ as brain,heart,liver,spleen,lung,kidney,skin,muscles and serum）. The results showed that the DI of model group was decreased compared with that of the control group（ P〈0. 01）,though the number value of AGEs,RAGE,sRAGE and esRAGE of each tissues in mice were higher than that of control（ P〈0. 01）. This experiment investigated that it could make organism in a state of high glycosylation when they were injected by D-galactose（ 200 mg kg^-1 d^-1） for 45 days. The model could be used in the study of the etiopathogenesis of AGEs which could discover the receptors and the pathway of pharmaceutical drug. And the index changing form in multiple tissues would give the researchers some suggestions in experimental targets using the model.