中文摘要：

目的：探讨砷暴露对不同发育阶段小鼠仔鼠海马CaMKⅡ表达水平的影响及可能的机制。方法：建立不同发育阶段小鼠仔鼠砷暴露模型（0、15、30和60 mg/L Na AsO_2组）,分别于仔鼠出生后（postnatal day,PND）第10、20和40天取脑组织并分离海马,采用real-time RT-PCR法检测CaMKⅡ亚单位CaMKⅡα和CaMKⅡβmRNA的表达水平。结果：PND10和PND20,不同剂量砷暴露组仔鼠海马CaMKⅡα和CaMKⅡβmRNA表达水平与对照组比较差异无统计学意义;PND40,不同剂量砷暴露组仔鼠海马CaMKⅡαmRNA表达水平均显著低于对照组,30和60 mg/L Na AsO_2组仔鼠海马CaMKⅡβmRNA表达水平均明显低于对照组（P〈0.05）。结论：砷暴露可使小鼠仔鼠海马CaMKⅡ亚单位mRNA的表达水平降低,从而可能影响仔鼠的学习记忆能力。

英文摘要：

Objective：To investigate the impairment mechanism of learning and memory ability induced by arsenite through studying the effects of arsenite on the expression level of CaMKⅡ in the hippocampus of offspring mice at different developmental stages. Methods：The model of offspring mice exposed to arsenite （0,15,30 and 60 mg/L NaAsO2） was set up. In postnatal day （PND） 10,20 and 40,the expression levels of CaMKⅡαand CaMKⅡβmRNA in the hippocampus were measured by real-time RT-PCR. Results：In PND 10 and PND 20, the expression levels of CaMKⅡ α and CaMKⅡ β mRNA in the hippocampus of offspring mice exposed to arsenite had no significant difference compared with those in control group. In PND 40, the expression levels of CaMKⅡα mRNA in the hippocampus of offspring mice exposed to arsenite were significantly lower than those in control group, the expression levels of CaMKⅡβ mRNA in the hippocampus of offspring mice exposed to 30 and 60 mg/L arsenite were significantly lower than those in control group （P〈0.05） . Conclusion：Arsenite can affect the expression levels of CaMKⅡsubunits in the hippocampus of offspring mice and further may impair the learning and memory ability.