中文摘要：

目的：确定FoxM1对HaCaT细胞增殖及细胞周期的影响.方法：采用DNA重组技术构建FoxM1的表达载体pEGFP-N2-FoxM1,脂质体法转染HeLa细胞并将其上清液按原浓度、1：1、1：2稀释后作用于HaCaT细胞.采用MTT法检测HaCaT细胞增殖情况;采用流式细胞仪测定HaCaT细胞周期变化.结果：FoxM1促进HaCaT细胞的增殖,随着浓度增加,增殖作用愈明显;HaCaT细胞经FoxM1作用后,G1期细胞比例显著降低,S期与G2期比例则显著升高.结论：FoxM1对HaCaT细胞具有诱导增殖作用,呈剂量依赖性.

英文摘要：

Objective：To determine the effects of FoxM1 on proliferation and cell cycle of HaCaT cells.Methods：Expression vectors of pEGFP-N2-FoxM1 were constructed and HeLa cells were transfected with lipofectamine.HaCaT cells were re-cultured with different concentration of supernatant from cultured HeLa cells.The proliferation of HaCaT cells was detected by MTT assay.The cycle of HaCaT cells was detected by by flow cytometry.Results：FoxM 1 can promote proliferation of HaCaT cells and the higher the concentration,the more obvious the proliferation effect was.The proportion of cells in phase G1 significantly reduced,and the proliferation of cells in phase S and G2 significantly increased after re-culture with FoxM1.Conclusion：FoxM 1 induces the proliferation of HaCaT cells in a dose dependent manner.