中文摘要：

目的：观察不同稀释度电针胃经后血清对大鼠胃黏膜细胞表皮生长因子受体表达的影响及其浓度效应相关性。方法：实验于2005-07／08在湖南中医药大学针灸基础实验室完成。①选择健康SD大鼠48只，随机分为1／2稀释度血清组、1／5稀释度血清组、1／10稀释度血清组和1／20稀释度血清组，每组12只。②随机选4只大鼠作为血清供体，采用华兴邦动物穴位谱并结合模拟人体经穴法进行取穴，足三里：膝关节后外侧，在腓骨小头下约5mm处。梁门：腹正中线与乳头线之间的中线上，脐上4寸。四白：眶下缘正中。电针刺激用G6805型电针仪，疏密波，电针频率疏波4Hz，密波20Hz，强度以肌肉微颤为度，电针时间30min。将针刺后大鼠行颈动脉取血，离心后吸取血清，将同组4只大鼠血清混合。③另8只大鼠不进行干预。利用链霉蛋白酶消化法分离大鼠胃黏膜细胞。④各组分别用1／2，1／5，1／10，1／20稀释度的血清孵育胃黏膜细胞，采用免疫细胞化学法检测胃黏膜细胞表皮生长因子受体表达水平。结果：纳入动物48只，均进入结果分析。1／2，1／5，1／10和1／20稀释度血清组大鼠胃黏膜细胞表皮生长因子受体均有较强表达，其中1／10稀释度血清组大鼠胃黏膜细胞表皮生长因子受体表达的平均灰度值和面积百分比与1／2，1／5，1／20稀释度血清组比较差异有显著性[平均灰度值：145．43±5．28，166．35±9．62，161．38±6．11，156．53±4．15；面积百分比：（21．62±2．08）％，（15．85±1．35）％，（18．07±1．28）％，（19．60±1．10）％，P〈0．05-0．01]。结论：电针胃经后血清可增强胃黏膜细胞表皮生长因子受体的表达，并且存在一定的浓度-效应相关性。

英文摘要：

AIM： To observe the effect of different diluted serum after electroacupuncture at gastric meridian on gastric mucosal cell epidermal growth factor receptor （EGFR） expression in rats and the relevance of density and effect. METHODS： The experiment was conducted at the Acupuncture and Moxibustion Laboratory of Hunan University of Traditional Chinese Medicine from July to August 2005. ①Totally 48 rats were randomly divided into 1/2 stomach meridian serum group （SMS）, 1/5 SMS group, 1/10 SMS group and 1/20 SMS group with 12 rats in each group; ②A total of 4 rats in each group were selected at random as the serum donors. Acupoints location was defined by reference of rat-acupoint-atlas and analogy to human body. According to the induction stated above, three pairs of acupoints consisting of Sibai （ST 2） （central infraorbital margin）, Liangmen （ST 21） （Central line between abdomen midline and mamillary line, 4 Chinese inch above belly button）, Zusanli （ST36） （back lateral knee joint, about 5 mm down fibula capitulum）, in the Foot Yangming Meridian, were designed. The needles were connected to the output of an electronic pulse generator, a medical electmacupuncture stimulator （Model G6805）, which achieved intermittent-and-irregular wave （intermittent wave： 4 Hz, irregular wave： 20 Hz, constant time of 30 minutes）, while there was a light vibration in the rats＇ lower limbs. The blood was sampled from arteria carotis after rats in each group were treated according to the requirement of experimental procedures, after centrifuged, the serum was carefully sucked by suction pipe, and the serum in the same group were mixed. ③The other 8 rats in each group were not intervened, and the gastric mucosal cell were separated by pronase digestion method. ④The gastric mucosal cell in each group were incubated by 1/2, 1/5, 1/10 and 1/20 diluted serum after electroacupuncture at stomach meridian. The EGFR expression on gastric mucosal cell was detected by immunocytochemistry meth