瞄准:在 Foot-Yangming ( SMFY )的胃顶点的 acupoints 调查电镀物品针灸( EA ),胃的粘膜的肠的翘摇上的 Foot-Yangming ( SMFY )的胆囊顶点在有胃的粘膜的导致压力的老鼠的因素( ITF )基因表示察觉损害,并且探索EA相关的胃的粘膜的规章的机制和意义保护的效果。方法:四十只老鼠随机被划分成 4 个组:空白的组,为组建模,在 SMFY 组(“ SMFY 组”) 的 acupoints 为 group+EA 建模,并且为在 GMFY 组(GMFY 组) 的 acupoints 的 group+EA 建模。所有老鼠(除了空白的组) 走水路被成为模型沉浸和抑制应力(WRS ) 。然后,在每只老鼠的胃粘膜织物在对胃的粘膜损害索引(图形用户界面) 的评价以后被脱掉,并且纸巾的 ITF mRNA 的表示被反向的抄写聚合酶检测链反应(RT-PCR ) 方法。结果:与模型组相比(54.3+/- 1.34 ),在 SMFY 的图形用户界面价值组织(31+/- 2.21 )显著地减少了( P【 0.01 ),那么做了在 GMFY 组织(39.8+/- 1.62 , P【 0.05 ),同时,在 SMFY 的图形用户界面价值组织比在 GMFY 组( P【 0.01 )显著地低。与模型组相比(0.65+/- 0.01 ) , EA 有一个趋势改进胃的粘膜 ITFmRNA 的表示基因:如此的趋势在 GMFY 组存在(0.66+/- 0.01 ) 但是没有有效差量(P】0.05 ) ,在 SMFY 组(0.76+/- 0.01 ) 与极其明显的差别(P【 0.01 ) ,而且,在 SMFY 的表示组织比在 GMFY 组(P【 0.01 ) 显著地高。结论:由在 SMFY 和 GMFY 的 acupoints 的 EA 的胃的粘膜 protective 效果与 ITF 的表示变化有关,显示某些顶点特性存在。它能是为 TCM 理论的一个证明“在 SMFY 和胃之间的相对个性”。
AIM: To investigate electroacupunture(EA) at the acupoints of Stomach Meridian of Foot-Yangming(SMFY), Gallbladder Meridian of Foot-Yangming(SMFY) on gastric mucosal intestinal trefoil factor (ITF) gene expression detection in stress-induced rats with gastric mucosal lesion, and to explore the regulatory mechanism and significance of EA-related gastric mucosal protective effect. METHODS: Forty rats were randomly divided into 4 groups: Blank group, Model group, Model group+EA at acupoints of SMFY group("SMFY group"), and Model group+EA at acupoints of GMFY group(GMFY group). All rats (except blank group) were made model by water immersion and restraint stress (WRS). Then the gastric mucosa tissue in each rat was taken off alter assessment of gastric mucosal lesion index(GUI), and the expression of ITF mRNA of the tissues was detected by reverse transcdption-polymerase chain reaction(RT-PCR) method. RESULTS: Compared with Model group(S4.3± 1.34), the GUI value in SMFY group (31±2.211 decreased significantly(P〈0.01), so did that in GMFY group (39.8± 1.62, P〈0.05), meanwhile GUI value in SMFY group was significantly lower than in GMFY group(P〈0.01). Compared with Model group (0.65±0.01), EA had a tendency to improve the expression of gastric mucosal ITFmRNA gene: such tendency existed in GMFY group (0.66±0.01) but with no signficant difference(P〉 0.05), in SMFY group(0.76±0.01) with an extremely obvious difference (P〈0.01), furthermore the expression in SMFY group was significantly higher than in GMFY group (P〈 0.01). CONCLUSION: The gastric mucosal protective effect by EA at the acupoints of SMFY and GMFY was related to the expression variance of ITF, indicating certain meridian specificity exists, It could be one proof for the TCM theory "Relative pardcularity between SMFY and stomach".