中文摘要：

目的 筛选对人气管上皮细胞株16HBE芳香烃受体基因mRNA表达有效抑制的特异RNAi片段。方法 自行制备内参照为竞争模板，应用定量竞争RT-PCR方法，分别检测转染有4个不同芳香烃受体基因RNA干扰位点的16HBE细胞芳香烃受体mRNA的表达，评价不同片段的RNA干扰效果。结果 转染4种芳香烃受体基因RNA干扰片段的16HBE细胞的每40ng总RNA芳香烃受体基因mRNA平均表达量分别为5．65fg、14．78fg、3．14fg和0．68fg，mRNA表达平均抑制率分别为61．6％、-0．5％、78．6％和95．4％。结论 应用定量竞争RT-PCR准确地定量基因的mRNA表达水平，筛查出芳香烃受体基因RNA干扰有效序列，为进一步研究芳香烃受体基因的功能创造了条件。

英文摘要：

Objective To evaluate and screen the specific RNAi fragments which can effectively inhibit Aryl hydrocarbon receptor（AHR） gene mRNA expression in human bronchial epithelial cell line （16HBE）. Methods AHR mRNA of 16HBE cells transfected 4 different AHR gene interfere sites were determined quantitatively with the quantitative comoetitive RT-PCR by using self-preoared internal standard as comoetitive temolates, and the RNA interfere effect was evaluated. Resets AHR ,nRNA average expression per 40ng total RNA of 16HBE cells transfected 4 different AHR gene interfere fragments were 5.65fg, 14.78fg, 3. ldfg and 0.68fg respectively, the average rates of inhibition were 61.6%, -0.5%, 78.6% and 95.4% respectively. Conclusion AHR gene specific effective RNA interfere sequence ware screened by quantitative competitive RT-PCR which could accurately quantify gene mRNA level, and offered condition for studying the gene function of AHR.