以新鲜杨梅渣为原料,利用正交试验法确定酸水提取杨梅渣红色素最佳工艺条件为料液比1:12(g/mL)、提取温度60℃、提提时间4h、pH1.5盐酸。采用离子交换树脂法对色素进行精制,在供试的5种树脂中D002的精制效果最好;通过静态吸附、解吸实验以及动态吸附实验,确定D002离子交换树脂精制杨梅渣红色素的工艺条件为杨梅渣红色素吸光度0.5~1、色素pH2~3、25℃左右、吸附时间1.5h、上样流速3BV/h。
An orthogonal array design was used to optimize conditions for the acidic extraction of red pigment from Myrica rubra pomace and optimum conditions for refining the crude pigment extract by ion exchange resin adsorption were studied. The optimum conditions for extracting red pigment from Myrica rubra pomace were found to be extraction at 60 ℃ for 4 h with acidic water at pH 1.5. D002 ion exchange resin showed the best refining efficiency for the crude pigment extract among 5 types of ion exchange resin investigated. Static adsorption and desorption and dynamic adsorption experiments were carried out to determine the optimum refining conditions as follows: sample concentration adjustment to an absorbance value (at 509.5 nm) varying from 0.5 to 1, pH adjustment to a value between 2 and 3, adsorption at around 25 ℃ for 1.5 h, and sample loading at a flow rate of 3 BV/h.