中文摘要：

目的：构建删除型微管驱动蛋白（KIF）11与麦芽糖结合蛋白（MBP）的融合蛋白的原核表达载体，并纯化得到高纯度的MBP-删除型KIF11融合蛋白。方法：PCR法从全长的KIF11基因组中扩增KIF11的相关删除型基因并连接到原核表达载体pMal中，此载体包含一个表达MBP的基因，删除型KIF11片段插入后与其融合，筛选和测序鉴定阳性克隆。将重组质粒转化到Rosseta大肠杆菌中，经异丙基硫代-β-D-半乳糖苷诱导表达和亲和层析分离纯化表达产物，对纯化的蛋白进行十二烷基磺酸钠-聚丙烯酰胺凝胶电泳法（SDS-PAGE）和蛋白质免疫印迹杂交（Westernblot）鉴定。结果：成功扩增删除型KIF11基因，构建MBP-KIF11-head，-stalk和-tail重组质粒并在Rosseta中诱导表达出MBP-KIF11-head，-stalk，-tail融合蛋白。经SDS-PAGE和Westernblot法验证了高纯度纯化的融合蛋白。结论：建立了高效稳定的MBP-KIF11表达体系，为进一步研究KIF11与mRNA调控蛋白锌指结合蛋白1相互作用的区域打下基础。

英文摘要：

Objective： To recombine truncated Kinesin superfamily protein （KIF） 11 gene in E.coli cells, and to purify the different fusion proteins of maltose binding protein （MBP）-KIF11. Methods： KIF11 cDNA was amplified by PCR. The KIF11 eDNA was inserted into the plasmid pMal. There was a MBP gene in pMal. By screening and sequenceing the recombinant plasmid, the positive constructs were transformed into the Rosseta host cells and induced by isopropyl-13-d-thiogalactoside. The recombinant products were purified in affinity chromatography and identified in sodium dodecyl sulfate polyacrylamide gel electrophoresis （SDS-PAGE） and Westem blot methods. Results： The truncated K/Fll gene was obtained successfully. The recombinant plasmid MBP-KIF 11-head, -stalk, -tail were constructed respectively. By SDS-PAGE and Westem blot methods, the fusion proteins MBP-KIFll-head, -stalk, -tail were purified successfully. Conclusion： The efficient prokaryotic expression system of MBP-KIF 11 is established. The purified MBP-KIF 11 protein provides to further study of the interaction between KIF 11 and mRNA regulation protein ZBP1.