中文摘要：

目的：探讨针刺作用的机理中是否存在着体液因素。方法：取新生大鼠心肌细胞进行原代培养。5天后应用荧光分子探针Fluo-3AM染色。激光共焦扫描显微镜动态扫描观察，加入正常血清和电针刺“内关”“间使”的血清后，观察心肌细胞内Ca^2＋含量的变化。结果：加入正常血清后，细胞内的Ca^2＋含量会有一定程度的升高，经过一段时间后升高的Ca^2＋含量会趋向平稳；而再加入针刺血清后，可明显降低心肌细胞Ca^2＋的含量（P〈0．01）。结论：针刺腧穴后的大鼠血清可使心肌细胞内Ca^2＋含量明显降低，为针刺效应的血清学机制提供了直接证据。

英文摘要：

Objective To explore whether humoral factors play a role in the mechanisms of acupuncture. Methods Primary culture of myocardial cells of neonatal rats were carried out. Five days later, they were labeled by fluorescent molecular probe Fluo-3AM. Changes of Ca^2＋ contents in the cultured myocardial cells after addition of the normal rat serum or acupuncture-serum of the rat who received acupuncture at ＂Neiguan＂ （PC 6） and Jianshi （PC 5）, were dynamically observed by a confocal laser scanning microscopy. Results After addition of normal serum of the rat, the intracellular Ca^2＋ level increased to a certain degree, and then gradually tended to stability, which was significantly decreased by addition of the acupuncture-serum （P〈0.01）, Conclusion The serum of the rat who received acupuncture at aeupoints can decrease the Ca^2＋ level in cultured myocardial cells, which provides a direct evi dence for serum factors involving in acupuncture mechanism.