借助"中国首次环球科学考察"航次,在东太平洋表层海水进行了添加氮、磷的现场培养实验。现场记录了实验水体温度的变化,用分光光度法对水体硝酸氮和活性磷酸盐浓度进行了检测,并用荧光法分析了水体叶绿素a浓度。结果表明,氮的添加会引起水体中叶绿素a浓度短期内快速增大,同时伴随硝酸盐浓度的显著降低,而单独添加P对水体中叶绿素a浓度影响并不显著;水体中N/P比值与叶绿素a浓度、N/P比值与浮游植物生长速度、温度与叶绿素a浓度以及温度与浮游植物生长速度之间均缺乏相关性。因此认为,在东太平洋实验海区表层海水中添加氮会引起浮游植物快速爆发,而磷的添加并不能刺激浮游植物快速生长,水体N/P比值和水体温度都不能单独控制浮游植物群落的生长。
Nutrient enrichment experiment was carried out in the East Pacific Ocean during the Chinese First Around-world Researching Cruise, with the addition of nitrogen or phosphorus to surface seawater. The variation of water temperature was recorded. The nutrient concentration was analyzed with spectrophotometry, and chlorophyll a concentration with fluorescence analysis. The addition of nitrogen resulted in the rapid increase of chlorophyll a concentration with the depletion of nitrogen in the experimental water, but no apparent variation occurred in chlorophyll a concentration when phosphate was added. The correlation was absent between the ratio of nitrogen to phosphorus and chlorophyll a concentration, the ratio of nitrogen to phosphorus and phytoplankton growing rate, water temperature and chlorophyll a concentration, and water temperature and phytoplankton growing rate. So it was considered that addition of nitrogen could stimulate phytoplankton bloom in the surface seawater in the East Pacific Ocean, while addition of phosphate could not. In addition, the ratio of nitrogen to phosphorus and the water temperature cannot control the growth of phytoplankton community.