中文摘要：

利用组成型卡那霉素抗性基因启动子（Pkan）,在肺炎克雷伯氏菌（Klebsiella pneumoniae）中过表达大肠杆菌醛脱氢酶基因ald H,获得重组菌K.p（p ETPkan-ald H）。微氧摇瓶发酵表明,该重组菌可高效利用甘油生产3-羟基丙酸,产量为0.47 g/L,较野生菌提高89.5%,甘油转化率、单位菌体产量分别提高100%和92%。5 L发酵罐微氧流加发酵表明,该菌3-HP产量达15.28 g/L,甘油转化率、产率分别为13.02%、12.73%。

英文摘要：

The aldehyde dehydrogenase gene aldH from E. coli has been subcloned into a vector under the promoter of kanamycin-resistance gene. Transformation of this vector into KlebsieUa pneumoniae DSM2026 resulted in a re- combinant strain named K. p（pETPkan-aldH）. Under micro-aerobic and shake-flask conditions, this recombinant strain produced 0.47 g/L of 3-hydroxypropionic acid （3-HP） , which was an increase of 89.5% when compared with the wild-type strain. The glycerol conversion ratio and yield on a biomass basis （YP/X） increased by 100% and 92% , respectively. More importantly, fed-batch fermentation in a 5 L bioreactor showed that this recombinant strain generated up to 15.28 g/L of 3-HP.