中文摘要：

克隆了肺炎克雷伯氏菌（Klebsiella pneumoniae）及大肠杆菌（Escherichia coli）各自的醛脱氢酶基因puuC和aldH,获得了重组肺炎克雷伯氏菌K.p（pET-PK-puuC）和K.p（pET-PK-aldH）,这两株菌均能以甘油为唯一碳源生产3-羟基丙酸（3-HP）.发酵实验结果显示K.p（pET-PK-puuC）的甘油转化率和单位菌体产量与野生菌相比分别提高了135％和18％；K.p（pET-PK-aldH）的3-HP产量达1.4.3 g/L,是K.p（pET-PK-puuC）的1.8倍,比野生菌提高了81％,甘油转化率和单位菌体产量比野生菌分别提高了150％和88％.

英文摘要：

The aldehyde dehydrogenase coding genes puuC and aldH from Klebsiella pneumoniae DSM 2026 and Escherichia coli K-12 respectively have been cloned, and two recombinant strains K. p （pET-PK-puuC） and K. p （pET-PK-aldH） were acquired. Upon glycerol induction, both strains could produce 3-hydroxypropionie acid （3- HP） using glycerol as the sole carbon source. Compared with the wild type strain, the reeombinant strain K. p （pET-PK-puuC） showed increases of 135% and 18% in glycerol conversion ratio and yield on a biomass basis, re- speetively. The strain K. p（pET-PK-aldH） produced 1.43 g/L of 3-HP, which is 1.8 times that produced by K. p （pET-PK-puuC）, and demonstrated increases of 81% , 150% and 88% in 3-HP production, glycerol conversion ratio and yield on a biomass basis, respeetively.