中文摘要：

应用DNA芯片研究禽致病性大肠杆菌可能致病基因的表达。构建禽致病性大肠杆菌毒力基因、潜在毒力基因的DNA芯片，应用基因芯片技术对同属02血清型的禽高致病性大肠杆菌E058株和低致病性大肠杆菌E526株在体外LB培养基和鸡血清培养状态下进行差异表达分析。结果：在体外LB静置培养状态下，低致病株E526与高致病株E058相比共有16个差异基因，均为下调基因。在鸡血清静置培养中，E526与E058相比共有15个差异基因，均为下调基因。应用基因芯片成功筛选了禽致病性大肠杆菌在体外不同条件下的毒力基因及可能毒力基因中差异表达基因，表明一些铁摄取系统相关基因对APEC的毒力较重要，同时也筛选出了一些新的可能致病基因aes-1，aes-2，aes-3，aes-4，aes-6，aes-8，aes-10，aes-13，aes-15，aes-31等。

英文摘要：

We constructed avian pathogenic Escherichia coli （APEC） specific DNA microarray to analyze the transcriptomes of APEC highly pathogenic strain E058 and low pathogenic strain E526 （both belonging to 02 serotype）. For cultures in Luria-Bertani （LB） broth medium, 16 distinctly expressed genes were observed in strain E526, and all of them were down-regulated. For cultures in the serum of chickens, 15 distinctly expressed genes were screened in strain E526, and all of them were also down-regulated. The results suggest that DNA microarray could be used to screen distinctly expressed genes among virulence- and potential virulence-associated genes of APEC. The expression of 11 common virulence- or potential virulence-associated genes were down-regulated for strain E526 compared to those of strain E058 cultured both in LB broth and chicken serum. Meanwhile, 4 potential virulence-associated genes,aes-3,aes-lO,aes-13 and aes-15 were down-regulated for strain E526 but not for strain E058, when they grew in the chicken serum. Besides known virulence factors, we observed 10 new potential virulence-related genes including aes-1, aes-2, aes-3, aes-4, aes-6, aes-8, aes-lO, aes-13, aes-15 and aes-31.