应用节杆菌(Arthrobacter spp.)K7和K3菌株菌悬液处理K326的采收后烟叶,并测定烘烤后烟叶烟碱含量。结果表明,烟叶经节杆菌菌悬液处理后,烟碱含量均有不同程度的降低,在田烟上使用效果略好于地烟,K7菌株对田烟的降烟碱效果好于K3菌株。K7和K3菌株菌悬液处理分别使田烟下部叶烟碱含量降低39.7%和34.9%,上部叶烟碱含量降低29.5%和29.2%,中部叶烟碱含量降低28.4%和26.1%。对各处理单料烟的评吸结果表明:节杆菌处理后,烟叶的香气质、香气量增加,烟气细腻,刺激性减轻,劲头有所下降且余味改善。两菌株对中部叶香气质的影响最大。
The harvested tobacco leaves of K326 were treated with bacterial suspension of strain K7 and K3 (Arthrobacter spp. ) and their nicotine contents were analyzed after flue-curing. The results indicated that the nicotine contents reduced in all treatments comparing with the control, the reduction rate in tobacco leaves grown in paddy field was slightly higher than that grown in hillside land, and the reducing effect of K7 was better than that of K3. For tobacco grown in paddy field, the reduction rates in lower, upper and middle leaves treated with K7 were 39.7% , 29.5% and 28.4% , respectively, and those treated with K3 were 34.9%, 29.2% and 26. 1%, respectively. The sensory evaluation of cigarettes made of each single grade tobacco showed that bacterial suspension could increase the aromatic quality and volume of tobacco leaf (especially for middle leaves) , reduce irritation and strength, and improve aftertaste.