中文摘要：

目的比较不同剂量髓鞘少突胶质细胞糖蛋白（myelin oligodendrocyte glycoprotein,MOG35-55）免疫诱导C57BL/6小鼠实验性自身免疫性脑脊髓炎（experimental autoimmune encephalomyelitis,EAE）的作用。方法将C57BL/6小鼠分为正常组和三组不同剂量MOG35-55诱导的EAE模型组,共4组。模型组分别以每只200、100、50μg的MOG35-55与完全弗氏佐剂（complete Freund s adjuvant,CFA）混合的乳化抗原皮下注射免疫诱导EAE模型,正常组以生理盐水替代。观察不同剂量MOG35-55对C57BL/6小鼠体重、发病率以及神经功能评分等影响,同时取小鼠脑和脊髓,利用光镜和透射电镜观察小鼠病理组织学改变。结果三组不同剂量MOG35-55均能诱导EAE模型,发病率为100%,呈慢性单相病程,病理学观察发现小鼠脑和脊髓有炎性细胞浸润、脱髓鞘及轴突损伤等改变。但小剂量组在体重减轻、临床症状评分及病理学改变等方面均较中、大剂量组明显。结论用MOG35-55 50μg剂量免疫诱导的C57BL/6小鼠EAE模型稳定,可在今后的研究中应用。

英文摘要：

Objective To compare the condition of illness and pathological characteristics of experimental autoimmune encephalomyelitis（EAE） in C57BL/6 mouse models induced by myelin oligodendrocyte glycoprotein 35-55（MOG35-55） at different doses,and provide a reliable animal model for further study of multiple sclerosis（MS）.Methods Male SPF-grade C57BL/6 mice were divided randomly into four groups： normal group and three EAE model groups（MOG35-55 high-dose,middle-dose and low-dose model groups）.200,100,50 μg MOG35-55/mice were mixed with complete Freund＇s adjuvant（CFA）,respectively,to prepare complete antigen in different concentrations.The mice were anesthetized and injected s.c.over flanks with the complete antigen and injected i.p.with pertussis toxin to establish immunizationinduced C57BL/6 mouse-model of EAE.The mice of the normal group were injected with normal saline instead.Since the day of immunization,the incidence,body weight and neurological score of the mice were observed.The mice of different neurological scores in different periods were anesthetized and perfused with saline and followed by 4% paraformaldehyde.The brain and spinal cord of the mice were removed and fixed in the same fixative solution.The brains and spinal cords of the mice were examined by histopathology with hematoxylin-eosin（HE） staining.The mice on the 40th day were sacrificed and perfused with 2% paraformaldehyde and 2% glutaraldehyde,1 mm~3 pieces of cerebral white matter and intumescentia lumbalis of the spinal cord were taken and ultrathin sections were prepared according to conventional techniques for electron microscopy.Results All the MOG_（35-55） in three different doses induced mouse models of EAE.The disease was with an incidence rate of 100% and a chronic monophasic course.The body weight of the mice in the three groups decreased obviously compared with those in the normal group.The maximum value of neurological score was 1.33,2.25 and 2.50 in the mice of high-,middle-and low-dose groups,respectivel