中文摘要：

学习 Zuogui 药片的分子的机制的目的(宸﹀?涓？ZGP ) 并且 Yougui 药片(鍙冲?涓？YGP ) 在在有试验性的自体免疫的脑脊髓炎(EAE ) 的老鼠的 axonal 新生上。方法 EAE 老鼠模型被抗原的下的注射在 1:1 的体积比率由混合髓磷脂使基本蛋白质(MBP ) 和完全的弗罗伊德鈥檚 助手(CFA ) 成为了的双边的后面的 pedes 建立。在大脑和针的绳索的 axonal 损害和新生的病理学的变化被观察在上第 14 (尖锐阶段) 并且在当模特儿以后的第 28 天(宽恕阶段) ，与 hematoxylin 曙红(他) 染色，银污点，并且 immunohistochemical 染色。与泼尼松醋酸盐对待的老鼠作为控制被拿。在轻显微镜与下面的结果观察他染色在老鼠鈥?显示出一个像袖子的变化有在小容器和 neuronic 变性作用附近的煽动性的房间渗入的脑髓的实质，当银染色显示出轴突的过多的肿胀和截去时，并且 immunohistochemical 分析出现了神经生长减少在 EAE 的尖锐舞台的因素( NGF )表示，在宽恕舞台甚至更显著，比较的出现重要差别并且 Nogo A，一个轴突生长禁止者，和它的受体的表情(Nogo-66 受体， Ng R ) 比在在尖锐舞台的正常控制的那些显著地高(P < 0.01 ) 。在 ZGP 和 YGP 的干预以后，然而，在老鼠鈥 ? 大脑和针的绳索的病理学的变化和轴突损坏是更减轻了，并且 NGF 表示在在尖锐舞台的模型组比那显著地高(P < 0.05 ) 。NGF 的表示在宽恕舞台期间是甚至更强壮的，并且更好的效果被 YGP 显示出。至于 Nogo A 和 Ng R 表情，他们是比在在尖锐阶段的模型组的那些显著地低的(P < 0.05 ) ，但是更好的效果被 ZGP 显示出。结论 ZGP 和 YGP 能阻止 axonal 损害并且在 EAE，和可能的机制的老鼠支持 axonal 新生是增加 NGF 的表示并且减少 Nogo A 和它的受体的表示。然而，一些差别在他们的代理时间和点在二中国准备之间被观察，它为在 tonifying 沈上揭?

英文摘要：

Objective：To study the molecular mechanism of Zuogui Pill（左归丸,ZGP） and Yougui Pill（右归丸,YGP） on axonal regeneration in rats with experimental autoimmune encephalomyelitis（EAE）.Methods： EAE rat model was established by bilateral rear pedes subcutaneous injection of antigen made by mixing myelin basic protein（MBP） and complete Freud＇s adjuvant（CFA） in the volume ratio of 1：1.The pathological changes of axonal injury and regeneration in the brain and the spinal cord were observed on the 14th（the acute stage） and the 28th day（the remission stage） after modeling,with hematoxylin-eosin（HE） staining,silver stain, and immunohistochemical staining.The rats treated with prednisone acetate were taken as controls.Results： Observation under the light microscope with HE staining showed a sleeve-like change in rats＇ cerebrospinal parenchyma with inflammatory cell infiltration around the small vessels and neuronic denaturation,while silver staining showed excessive tumefaction and abscission of axon,and immunohistochemical analysis showed decreasing of nerve growth factor（NGF） expression at the acute stage of EAE,which was even more remarkable at the remission stage,showing significant difference as compared with the normal control（P〈0.05）. And the expressions of Nogo A,an axon growth inhibitor,and its receptor（Nogo-66 receptor,Ng R） were significantly higher than those in the normal control at the acute stage（P〈0.01）.However,after the intervention of ZGP and YGP,the pathological changes and axon damage in rats＇ brain and spinal cord were much more alleviated,and the NGF expression was significantly higher than that in the model group at the acute stage （P〈0.05）.The expression of NGF was even stronger during the remission stage,and a better effect was shown by YGP.As for Nogo A and Ng R expressions,they were significantly lower than those in the model group at the acute stage（P〈0.05）,but a better effect was shown by ZGP.Conclusions：ZGP and