中文摘要：

目的探讨皮肤黑素瘤及痣细胞痣组织中钠氢交换子调节因子-1（NHERF1）和β-联蛋白表达的意义。方法收集47例皮肤黑素瘤及37例痣细胞痣组织，用免疫组化方法对NHERF1和β-联蛋白在组织中的表达模式（膜表达、浆表达及核表达）和表达强度进行检测。结果原位皮肤黑素瘤组织中NHERF1胞质中等服阳性表达率为38％；而侵袭性皮肤黑素瘤组织中NHERF1胞质中等／强阳性表达率为71％，两组相比差异有统计学意义。同时，全部皮肤黑素瘤组织中NHERF1胞质中等偶阳性表达率为60％，而痣细胞痣组织中NHERF1胞质中等／强阳性表达率为0，差异有统计学意义。原位皮肤黑素瘤组织中可见β-联蛋白胞质中等／强阳性表达率为50％；而侵袭性皮肤黑素瘤组织中可见β-联蛋白胞质中等，强阳性表达率为84％。两组相比差异有统计学意义。全部皮肤黑素瘤组织中β-联蛋白胞质中等／强阳性表达率为72％，而痣细胞痣组织中NHERF1胞质中等／强阳性表达率为30％，两组相比具异有统计学意义。NHERF1及β-联蛋白胞质中等／强阳性表达率在痣细胞痣组、原位黑素瘤组及侵袭性黑素瘤组中均随着病变组织的恶性程度增加而升高。运用Spearman秩相关检验对皮肤黑素瘤中NHERF1及B一联蛋白的表达相关性进行分析显示无相关性。结论NHERF1及β-联蛋白在痣细胞痣、原位黑素瘤及侵袭性黑素瘤组织中的胞质阳性表达随着病变组织的恶性程度增加而升高，但两者的表达差异无相关性。

英文摘要：

Abstraet Objective To detect the expression of Na＋/H＋ exchanger regulatory factor 1 （NHERF1） and β-catenin in cutaneous melanoma and melanocytic nevus tissues. Methods Tissue samples were obtained from the lesions of 47 patients with cutaneous malignant melanoma and 37 patients with melanocytic nevi. Immunohistochemistry was used to determine the expression pattern （membrane, cytoplasmic or nuclear expression） and intensity of NHERF1 and β-catenin. Results The percentage of tissue samples with moderate/strong cytoplasmic expression of NHERF1 significantly differed between cutaneous melanoma in situ and invasive melanoma （38% vs. 71%, P 〈 0.05）, as well as between cutaneous melanoma and melanocytic nevi （60% vs. 0, P 〈 0.05）. A moderate/strong cytoplasmic expression of β-catenin was observed in 50% of the cutaneous melanoma in situ tissue samples compared to 84% of the invasive melanoma tissue samples （P 〈 0.05）, and 72% of all the cutaneous melanoma tissue samples compared to 30% of the melanocytic nevus tissue samples （P 〈 0.05）. The moderate/strong cytoplasmic expression rate of both NHERF1 and β-catenin in these tissue samples increased with the increase in degree of malignancy （i.e., from melanocytic nevi, melanoma in situ to invasive melanoma）. Spearman＇s rank correlation test revealed no correlation between the expression of NHERF1 and β-catenin in cutaneous melamoma tissues. Conclusions The moderate/strong cytoplasmic expression rate of both NHERFI and β-catenin sequentially increases from melanocytic nevi, melanoma in situ to invasive melanoma, but there is no correlation between the expression of NHERF1 and β-catenin in cutaneous melamoma tissues. [Key words] Melanoma; Nevus; NHERF1; β-catenin; Immunohistochemistry