中文摘要：

我们在 SW480 人的 colorectal 癌症房间线调查了 MSH2，在房间增长的失配修理基因，房间周期控制和房间侵略的角色。MSH2 表示的调停 RNAi 的抑制用 MSH2 shRNA lentiviral 表示向量被完成。有效内长的 MSH2 击倒表示被即时 PCR 分析决定。最有效的 MSH2 击倒的向量用 SW480 房间为随后的研究被选择。内长的 MSH2 mRNA 层次在 MSH2-RNAi 的 lentiviral 交货以后减少了，显示在 SW480 房间的 MSH2 表示的有效 silencing。房间增长，房间周期前进和房间侵略被 MTT 试金，流动 cytometry 和 transwell 试金分别地确定。在 SW480 房间的 MSH2 表示的调停 RNAi 的抑制导致了减少的房间增长，在 G0/G1 阶段的房间周期拘捕和减少的房间侵略。一起拿，这些结果提供 MSH2 刺激房间增长，支持房间周期前进并且断然调整房间侵略的证据。

英文摘要：

We have investigated the role of MSH2,a mismatch repair gene in cell proliferation,cell cycle control and cell invasiveness in the SW480 human colorectal cancer cell line.RNAi-mediated inhibition of MSH2 expression was achieved using MSH2 shRNA lentiviral expression vectors.Effective knockdown of endogenous MSH2 expression was determined by real-time PCR analysis.The most efficient MSH2 knockdown vector was selected for subsequent studies using SW480 cells.Endogenous MSH2 mRNA levels decreased after lentiviral delivery of the MSH2-RNAi,indicating efficient silencing of MSH2 expression in SW480 cells.Cell proliferation,cell cycle progression and cell invasiveness were quantified by MTT assays,flow cytometry and transwell assays,respectively.RNAi-mediated inhibition of MSH2 expression in SW480 cells resulted in decreased cell proliferation,cell cycle arrest at the G0/G1 phase and decreased cell invasiveness.Taken together,these results provide evidence that MSH2 stimulates cell proliferation,promotes cell cycle progression and positively regulates cell invasiveness.