中文摘要：

作为引起水稻病害的主要病菌之一,稻瘟病菌是一种重要的模式生物,对于研究植物与病原菌之间的互作起着极其重要的作用。甘油激酶在体内可以催化甘油转化为甘油3-磷酸,对甘油的分解代谢起着重要的作用。在稻瘟病菌基因组中有两个编码甘油激酶的基因,分别为Mogly1和Mogly2。我们通过体内同源重组的基因敲除技术,获得两个基因的基因缺失突变体ΔMogly1和ΔMogly2,以及两个基因的双敲突变体ΔMogly1ΔMogly2。表型分析发现这些突变体在生长,产孢,致病性等方面相对于野生型均没有明显变化,但是ΔMogly1和ΔMogly1ΔMogly2在完全培养基上面的气生菌丝减少,菌落颜色变白。通过测量这些突变体在不同碳源培养基上的生长,发现ΔMogly1和ΔMogly1ΔMogly2在以山梨醇、葡萄糖、甘油和蔗糖为唯一碳源的培养基上生长速率均减慢。而在以甘油为唯一碳源的培养基上生长速度被抑制的最为显著。这说明Mogly1和Mogly2在碳源代谢中的作用是不同的,Mogly1基因可能在碳源代谢中扮演着重要的角色,而Mogly2可能是功能冗余的基因。

英文摘要：

As one of the major fungi causing rice diseases, Magnaporthe oryzae is an important model organism, which plays a very important role in the study of the interaction between plants and pathogens. Glycerol kinase can catalyze the conversion of glycerol to 3-Glycerol phosphate kinase, which is involved in the metabolism of glycerol. The Magnaporthe oryzae contains two glycerol kinases, encoded respectively by Moglyl and Mogly2 genes. Using the homologous recombination strategy, we obtained the gene knock-out mutants of Moglyl, Mogly2 and double disruption mutant. Disruption of Moglyl and Mogly2 did not cause any differences on the phenotype of growth, sporulation, pathogenicity. However, the colony of Moglyl has fewer aerial hyphae and the color was getting a little white. Moreover, the double disruption mutant-ZlMoglylAMogly2 has the same defect on the aerial hyphae as ZlMoglyl did. By measuring colony growth on different carbon sources, we found that the growth rate of zlMoglyl and AMoglylAMogly2 were significantly reduced on the medium with sorbitol, glucose, glycerol and sucrose as sole carbon sources. Moreover, they showed the greatest inhibited rate in the culture with glycerol as sole carbon source. Thus, zlMoglyl but not AMogly2 played an important role in the metabolism of carbon source in Magnaporthe oryzae. However, the Mogly maybe play a redundant role in M. oryzae.