中文摘要：

目的：探讨炎症微环境作用下牙周膜干细胞成骨分化能力的变化及糖原合成酶激酶3β（glycogen synthesis kinase,GSK3β）对其成骨分化能力的影响。方法：培养正常及慢性牙周炎组织来源的牙周膜干细胞（H-PDLSCs,P-PDLSCs），将两种PDLSCs体外成骨诱导7d，实时定量PCR检测细胞Runx2、 ALP、 OCN的基因表达水平， Western Blot检测p-GSK3β， GSK3β的蛋白表达情况。在成骨诱导时使用GSK3β特异性抑制剂LiCl刺激PDLSCs， ALP染色、 RealTime PCR检测PDLSCs成骨分化的情况。结果： P-PDLSCs的成骨化能力显著低于H-PDLSCs。 P-PDLSCs组p-GSK3β表达较H-PDLSCs组增高，成骨诱导后，两种PDLSCs中p-GSK3β表达降低，但GSK3β总蛋白表达水平增高。 LiCl抑制GSK3β后PDLSCs的ALP活性、 Runx2和OCN表达水平显著降低。结论：在慢性炎症微环境中， GSK3β的磷酸化可影响Wnt信号通路，抑制牙周膜干细胞的成骨分化。

英文摘要：

Objective：To investigate the osteogenic differentiation potential and the role of GSK3βof human periodontal ligament stem cells in inflammatory microenvironment. Methods： Periodontal ligament stem cells were obtained from human healthy individuals and patients with periodontitis （H-PDLSCs, P-PDLSCs）. P-PDLSCs and H-PDLSCs were cultured in osteogenic medium for 7 days. We tested the mRNA expression of Runx2、 ALP、 and OCN by Real time PCR, protein expression of p-GSK3βand GSK3βwere examined by Western Blot. Stimulated PDLSCs whih LiCl in osteogenic induction process, bone formation ability was tested by ALP staining and RealTime PCR. Results： The osteogenic differentiation potential of P-PDLSCs was significantly reduced than the H-PDLSCs.We found more strikingly enhanced p-GSK3βP-PDLSCs than H-PDLSCs. After osteogenic differentiation, the expressions of p-GSK3βwas down-regulated, but the total GSK3βexpression level was up-regulated in both two types of PDLSCs. Both the ALP staining and mRNA expression levels of Runx2 and OCN showed that the osteogenic differentiation of PDLSCs decreased significantly after LiCl stimulated. Conclusion：The phosphorylation of GSK3βcan affect the Wnt signaling pathway, which mediated the impaired osteogenic differentiation of PDLSCs in inflammatory microenvironments.