中文摘要：

目的：探讨炎症作用下Caspase-3/8对人牙龈间充质干细胞凋亡能力影响的研究,为探索牙龈炎的预防和治疗策略提供理论和实验依据。方法：取20~25岁拔除阻生齿牙切除的无炎症龈瓣和正畸过程中增生的牙龈组织,分离培养正常牙龈间充质干细胞（NGMSCs）和炎性牙龈间充质干细胞（IGMSCs）。Annexin V-FITC/PI双染检测相同培养条件下NGMSCs与IGMSCs凋亡率;应用PCR,免疫荧光法检测各组间Caspase-3,Caspase-8因子表达差异。结果：Annexin V-FITC/PI双染检测结果显示相同培养条件下IGMSCs凋亡率（10.44%）〉NGMSCs凋亡率（2.76%）。PCR结果显示IGMSCs组Caspase-3,Caspase-8表达显著高于NGMSCs组（P〈0.01）。IGMSCs组TNF-α,IL-1β基因表达显著高于NGMSCs组（P〈0.01）。结论：Caspase-3/8在牙龈间充质干细胞增殖、凋亡途径中起着重要调控作用。

英文摘要：

Objective：To explore the the effect of Caspase-3/8on apoptosis of human gingival mesenchymal stem cells under inflammation.Methods：Gingival tissues with or without inflammation were obtained from the third impacted molars and gingival hyperplasia because of orthodontic treatment,respectively.Patients were all about 20-25 years old.Normal gingival mesenchymal stem cells（NGMSCs）and inflammatory gingival mesenchymal stem cells（IGMSCs）were isolated and cultured.Apoptosis rates of IGMSCs and NGMSCs were determined by VFITC/PI Annexin double staining.Caspase-3and Caspase-8expression differences were detected by Real-Time PCR.Results：Apoptosis rate of IGMSCs（10.44%）was significantly more than that of NGMSCs（2.76%）.Expressions of Caspase-3and Caspase-8in IGMSCs group were significantly higher than those of NGMSCs group（P〈0.01）as well as TNF-αand IL-1βgene expressions.Conclusion：Caspase-3/8plays an important role in regulating the proliferation and apoptosis of mesenchymal stem cells.