中文摘要：

目的探讨GRIM-19 mRNA与蛋白的表达在结直肠癌发生、发展中的意义。方法采用逆转录．聚合酶链反应（RT-PCR）和免疫组织化学方法检测40例结直肠癌患者癌组织，正常组织中的GRIM-19 mRNA及蛋白表达情况及测序分析。结果结直肠癌组织中的GRIM-19 mRNA和蛋白水平明显低于正常组织，GRIM-19蛋白表达在癌组织中明显低于正常组织，且GRIM-19蛋白表达水平与结直肠癌分化程度和临床分期有关（P均〈0．05）。结论GRIM-19作为一种抑癌基因和分子标志物，其表达降低及失活可能与结直肠肿瘤的发生、发展有关。

英文摘要：

Objective To study the expression of grim-19 in colorectal carcinoma and its role in tumorigenesis. Methods The expression of grim-19 was detected by using RT-PCR and immunohistochemistry in the matched samples from 40 cases of malignant and normal tissues of colorectal tumors. Base sequences of the DNA product were determined. Results The expression level of grim-19 mRNA and pro- tein in malignant colorectal tumors was obviously lower than in normal tissues. The expression of grim-19 was correlated with clinical stage and cell differentiation of colorectal cancer （ P 〈 0.05 and P 〈 0.05 respectively）. Conclusion Grim-19 could be used as a novel tumor suppressor gene and molecular marker. Inactivation of grim-19 gene expression may be linked to oncogenesis.