中文摘要：

目的：研究干扰素/维甲酸诱导死亡基因（retinoid-interferon-induced mortality,GRIM-19）对结肠癌SW480细胞凋亡的影响。方法：构建GRIM-19真核表达载体（pCMV-Flag-GRIM-19）,转染入SW480细胞中,Western blotting检测GRIM-19及凋亡相关蛋白Bal-xl的表达,采用Annexin-V/PI和线粒体膜电位JC-1染色检测SW480细胞的凋亡。结果：成功构建pCMV-Flag-GRIM-19真核表达载体。pCMV-Flag-GRIM-19质粒转染SW480细胞后,GRIM-19的表达上调,凋亡抑制蛋白Bcl-xl的表达则下调。转染空质粒pCMV-Flag组SW480细胞凋亡率为（7.7±1.39）%,转染pCMV-Flag-GRIM-19质粒后SW480细胞凋亡率为（15.0±2.52）%（P〈0.05）。线粒体膜电位检测显示转染空质粒pCMV-Flag组SW480细胞膜电位降低细胞为（7.5±2.09）%,而转染pCMV-Flag-GRIM-19后细胞线粒体膜电位降低细胞为（17.5±3.07）%（P〈0.05）。结论：GRIM-19体外转染可有效诱导结肠癌SW480细胞凋亡。

英文摘要：

Objective：To investigate the effect of retinoid-interferon-induced mortality （GRIM-19） gene on the apoptosis of colon cancer.Methods：A GRIM-19 eukaryotic expression vector （pCMV-Flag-GRIM-19） was constructed and transfected into SW480 cells.Expressions of GRIM-19 and apoptosis-related proteins were detected by Western blotting analysis.Apoptosis of SW480 cells was measured by Annexin-V/PI assay and mitochondrial membrane potential JC-1 staining.Results：The GRIM-19 eukaryotic expression vector pCMV-Flag-GRIM-19 was successfully constructed.Expression of GRIM-19 in SW480 cells was up-regulated and that of apoptosis-related protein Bcl-xl was down-regulated after transfection with pCMV-Flag-GRIM-19.Apoptosis rate was （7.7±1.39）% in SW480 cells transfected with pCMV-Flag empty vector and （15.0 ± 2.52）% in pCMV-Flag-GRIM-19 transfected cells （P〈0.05）.Mitochondrial membrane potential was decreased in （7.5±2.09）% of pCMV-Flag transfected cells and （17.5±3.07）% of pCMV-Flag-GRIM-19 transfected cells （P〈0.05）.Conclusion：In vitro GRIM-19 transfection can effectively induce apoptosis of colon cancer SW480 cells.