中文摘要：

目的观察短发夹状干扰RNA靶向抑制Hax-1基因诱导HaCaT细胞凋亡的作用。方法构建特异性抑制Hax-1的shRNA表达载体并转染HaCaT细胞;分别采用半定量PCR以及Western blot从mRNA和蛋白水平检测干扰效果;流式细胞术检测HaCaT细胞的凋亡。结果 Hax-1基因mRNA水平以及蛋白水平的表达均得到显著抑制,HaCaT细胞凋亡率明显升高。结论靶向Hax-1的shRNA显著抑制了Hax-1基因在HaCaT细胞中的表达,并明显诱导HaCaT细胞凋亡。

英文摘要：

Objective To study the function of HS1-associated protein X-1（Hax-1）shRNA in inducing apoptosis of human keratinocyte line HaCaT.Methods ShRNA expression vector pSilencer-Hax-1 was constructed.Reverse transcription-polymerase chain reaction（RT-PCR） technique and Western blot were used to analyze mRNA and protein expressions of Hax-1 in transfected HaCaT cells.And then apoptotic HaCaT cells were detected by flow cytometry.Results Expression of the mRNA and protein of Hax-1 was down-regulated and obvious apoptosis of HaCaT was induced.Conclusion siRNA mediated by pSilencer-Hax-1 significantly inhibited the expression of Hax-1 in HaCaT cells and obviously induced the apoptosis of HaCaT cells.