以大白菜—结球甘蓝4号染色体单体异附加系自交后代为材料,通过根尖染色体计数法鉴定,从36株中筛选出3株2n=22的植株。染色体核型分析结果表明,该3株为大白菜—结球甘蓝4号染色体二体异附加系。该二体异附加系在生殖生长时期表现植株较矮小,基生叶卵圆形、绿色,茎生叶披针形、浅绿色,开花时间晚,黄花,花器官较大白菜—结球甘蓝4号染色体单体异附加系小,花粉量少、结荚率和结籽率低。减数分裂观察发现:后期Ⅰ染色体主要以11︰11分离方式为主,所占比率为70.59%;后期Ⅱ染色体以11︰11︰11︰11为主,其比率为69.57%。
The selfing progenies derived from Chinese cabbage–cabbage monosomic addition line 4 were used as materials,3 plants with 2n = 22 were selected by chromosome counting from 36 selfing progenies,and the results from karyotype analysis showed that the 3 plants were Chinese cabbage–cabbage disomic alien addition line introducing chromosome 4 of cabbage. During reproductive period,the disomic alien addition line plant was represented by weak vigor,ovate and green basal leaves,lanceolate and light green stem leaves,late flowering time,yellow flower petals,smaller floral organ size and decreased number of pollen grains compare with monosomic alien addition line. As well as the rate at which selfed silique and seed were set was lower. The chromosome distribution with 11︰11 at napheⅠ represented 70.59%,and the rate of chromosome distribution with 11︰11︰11︰11 was 69.57% at anaphaseⅡ.