中文摘要：

分析10 L发酵罐中地衣芽孢杆菌降解氯氰菊酯的分批培养动力学。以装料系数0.65、体积分数6.25%的接种量、初始pH 7.5、培养温度37℃、转速300 r/min为发酵条件,探讨地衣芽孢杆菌B-1分批培养过程中菌体生长、氯氰菊酯降解酯酶合成、底物消耗及氯氰菊酯降解的规律。在Logistic方程、Luedeking-Piret方程、Luedeking-Piret-Like方程及动力学一级模型的基础上进行非线性拟合,建立了菌株B-1菌体生长、氯氰菊酯降解酯酶生成、基质——葡萄糖消耗和氯氰菊酯降解动力学模型。将实验实测值与模型理论值进行比较,结果表明模型能较好地反映菌株分批培养过程的动力学特征。

英文摘要：

The kinetics of the batch fermentation of Bacillus licheniformis for cypermethrin degradation in a 10 L fermentor was analyzed. Cell growth, the production of cypermethrin-degrading esterase, substrate consumption and cypermethrin degradation were investigated when the fermentation conditions were as follows： filling coefficient, 0.65; inoculum amount, 6.25%（V/V）; initial pH, 7.5; temperature, 37 ℃; and rotation speed, 300 r/min. Kinetic models for the growth ofBacillus licheniformis B-1, cypermethrin-degrading esterase production, substrate（glucose） consumption and cypermethrin degradation were established through nonlinear regression using Logistic equation, Luedeking-Piret equation, LuedekingPiret-Like equation and first-order kinetic model. The results showed that the predicted values from these models were in good agreement with the experimental data and these models could provide a reasonable description for the batch fermentation process of Bacillus licheniformis B-1 strain.