中文摘要：

采用急性毒性实验方法,研究了镉（Cd^2＋）对河南华溪蟹（Sinopotamon henanense）精子质量的影响.实验设置了5个Cd^2＋浓度组（7.25、14.5、29、58和116mg·L^-1）和1个空白对照组,在2个染毒时间（5d和7d）采用特异性的荧光染料和流式细胞术（FCM）对精子成活率、膜完整性、顶体完整性及染色质结构进行了测定.结果显示,随着Cd^2＋浓度的增加和染毒时间的延长,精子的成活率下降,质膜和顶体缺失率上升,异染色质所占比率上升.在Cd^2＋浓度为58、116mg·L^-1条件下暴露5d后,精子成活率显著降低（p〈0.05）;Cd^2＋浓度为116mg·L^-1时,精子质膜完整性和染色质结构均有明显的损伤;而顶体状态在Cd^2＋浓度为29mg·L^-1时就呈现显著的损伤（p〈0.01）.在Cd^2＋浓度为29、58、116mg·L^-1条件下染毒7d后,精子成活率、质膜完整性和顶体完整性降低;在所有的染毒组,DNA结构受到了显著的破坏.结果表明,Cd^2＋暴露对华溪蟹精子质量有明显的影响.

英文摘要：

To explore the effects of acute cadmium （Cd^2 ＋ ） on the sperm quality of Sinopotamon henanense, the freshwater crabs Sinopotamon henanense were exposed to 0, 7.25, 14.5, 29, 58, and 116 mg·L^-1 Cd^2＋ for 5 and 7 d. Using specific fluorescent dyes and flow cytometry （FCM） , the sperm quality was investigated including sperm viability, plasma- and acrosomal- membrane integrity and chromatin integrity. Our results showed that with the increase of Cd^2 ＋ concentration and exposure timethe sperm viability decreased, plasma- and acrosomal- membrane loss increased and the ratio of abnormal DNA increased. When exposed to 58 and 116 mg·L^-1 Cd^2＋ for 5 d, the sperm viability was significantly reduced; sperm membrane integrity and chromatin structure were also significantly damaged by 116 mg·L^-1 Cd^2＋; the acrosomal integrity were also damaged starting at 29 mg·L^-1 Cd^2＋. When exposed to 29, 58, and 116 mg·L^-1 Cd^2＋ for 7 d, the sperm viability was significantly lowered and plasma- and acrosomal- membrane integrity were undermined. The chromatin structure, however, were damaged at all Cd^2＋ -exposed groups. Our results indicated that Cd2 ＋ exposure can damage sperm quality severely of the crab species.