在粳稻嘉花1号水稻品种经60 Coγ辐照后代中筛选到一个幼苗叶色温敏感突变体tsl11。与野生型嘉花1号相比,该突变体在20℃下,幼苗叶色呈黄色;叶绿素a、叶绿素b和类胡萝卜素含量分别下降94.6%、88.2%和91.5%;且电镜结果显示叶绿体内部结构不完整。而在高温(32℃)条件下突变体的叶色表型、光合色素含量和叶绿体发育与野生型基本一致,显示出明显的温度敏感性。遗传分析表明,该突变体叶色性状受1对隐性核基因控制,暂命名为tsl11(thermo-sensitive leaf-color11)。以该突变体与籼稻培矮64S杂交,构建4928株突变型F2,利用SSR和InDel分子标记将tsl11基因定位于水稻第11染色体上的ID14506与ID14653之间的147kb的区间内。
A thermo-sensitive leaf-color mutant, tsUl , was selected from the progeny of the japonica rice variety Jiahua 1 by 60Co )'-radiation. Compared with the wild-type parent Jiahua 1 at 200C, the mutant was characterized yellowseedling, decreased contents of chlorophyll a, chlorophyll b and carotenoid by 94.6 %, 88.2% and 91.5 %, respectively. Simultaneously, the result of electron microscopic observation revealed that the structure of chloroplasts in the tslll was imperfect seriously. However, the mutant was similar to wild type at high temperatures(32℃). These results indicated that the mutant was obviously thermo-sensitive. Genetic analysis showed that the mutant trait was controlled by a single nuclear recessive gene, tentatively designated as tsll 1. Moreover, the mapping population of 4928 F2 mutant individuals derived from Pei' ai 64S/tsl11 were constructed, then using SSR and InDel markers, the tslll gene was finally mapped to a region of 147 kb between ID14506 and ID14653 on chromosome 11.