中文摘要：

目的 观察叔丁基对苯二酚（tert_hutylhydroquinone，tBHQ）对皮肤角质细胞核因子Nrf2（transcriptionfactorNF—E2-relatedfactor2）及其调控的下游靶基因NAD（P）H醌氧化还原酶[NAD（P）H：quinoneoxidoreductase1，NQ01]和血红素单加氧酶-1（hemeoxygenase1，HO-1）蛋白表达的影响。方法向HaCaT细胞中加入终浓度为50txmol／L的tBHQ溶液分别培养0（对照）、2、6、12、24、36h；或分别加入终浓度为0（对照）、25、50μmol／L的tBHQ溶液培养24h。采用westemblot法检测细胞Nrf2、NQ01和HO-1的蛋白表达。结果与对照组比较，50μmol／L的tBHQ染毒2～36h时HaCaT细胞Nrf2蛋白表达水平，染毒12—36h时HaCaT细胞NQ01蛋白表达水平以及染毒6～36h时HaCaT细胞HO-1蛋白表达水平均升高，差异有统计学意义（P〈0．05或P〈0．01）。25μmol／L和50μmol／LtBHQ分别染毒24h后HacaT细胞的Nrf2、NQ01和HO-1的蛋白表达水平均高于对照组，差异有统计学意义（P〈0．01）。与25μmol／LtBHQ染毒组比较，50Ixmol／LtBHQ染毒组Nrf2和HO-1蛋白表达水平均下降，NQ01蛋白表达水平升高，差异有统计学意义（P〈0．01）。结论tBHQ能够诱导皮肤角质细胞的核转录因子Nrf2及其调控的下游靶基因NQ01和HO-1的蛋白持续表达，从而对皮肤角质细胞产生保护作用。

英文摘要：

Objective To observe the effects of tert-butylhydroquinone （tBHQ）on protein expressions of transcription factor Nrf2 and its regulated downstream target genes,NAD （P）H：quinone oxidoreductase 1 （NQO1）and heine oxygenase 1 （HO-1）in human keratinocytes. Methods Human keratinocyte HaCaT cell line was exposed to 50 μmoFL of tBHQ for 0 h （control）, 2 h, 6 h, 12 h,24 h and 36 h,or 0 μmol/L （control）,25 μmol/L and 50 μmol/L of tBHQ for 24 h. Total cellular protein was extracted and Western Blot assays were used to detect the protein expression of Nrf2,NQO1 and HO-1. Results Compared with control group,50 μmol/L tBHQ could significantly induced the protein expressions of Nrf2 （2-36 h）,NQO1 （12-36 11） and HO-1（6-36 h）, respectively （P〈0.05 or P〈0.01 ）. 25 and 50 μmol/L tBHQ could similarly significantly induced the protein expressions of Nrf2, NQO1 and HO-1 at 24 h（P〈0.01 ）. However,Nrf2 and HO-1 proteins in 50 μmol/L tBHQ treatment were decreased,while NQO1 proteins were increased remarkable than 25 μmoFL tBHQ treatment （P〈0.01）. Conclusion tBHQ may induce the sustained protein expressions of Nrf2 and its regulated downstream NQO1 and HO-1 ,which may provide the protective effects for the cells.