中文摘要：

瞄准：为了如果表明小径的 cGMP/cGMP-dependent 蛋白质激酶 G (PKG ) 可以参予树眼镜蛇属尿钠排泄的，系统地调查，肽(DNP ) 导致了胃的圆形的平滑肌的松驰。方法：在畿尼猪的 cGMP 的内容胃的窦平滑肌织物和灌注答案用放射性免疫测定被测量；用 4 隧道 physiograph 记录的胃的窦通报肌肉的自发性收缩；并且激活 Ca2+ 的 K+ 水流(IK (Ca )) 和在孤立的胃的窦 myocytes 的自发的短暂外面的水流(STOC ) 用整个房间的补丁 clamp 技术被记录。结果：DNP 显著地在胃的窦平滑肌组织并且在灌注媒介提高了 cGMP 层次。DNP 在胃的窦通报平滑肌导致了松驰，它被 KT5823 禁止，一个 cGMP 依赖的 PKG 禁止者。DNP 增加了 IK (Ca ) 。这效果被 KT5823 几乎完全堵住，并且部分由 LY83583 堵住了， guanylate cyclase 到的一个禁止者改变 cGMP 的生产。DNP 也增加了 STOC。STOC 上的 DNP 的效果面对 KT5823 被废除，然而并非由 KT-5720 影响了，一个 PKA 特定的禁止者。结论：DNP 激活 IK (Ca ) 并且放松豚鼠经由挑选轴而不是 cAMP/PKA 的 cGMP/PKG-dependent 的胃的窦通报平滑肌小径。

英文摘要：

AIM： To systematically investigate if cGMP/cGMPdependent protein kinase G （PKG） signaling pathway may participate in dendroaspis natriuretic peptide （DNP）-induced relaxation of gastric circular smooth muscle. METHODS： The content of cGMP in guinea pig gastric antral smooth muscle tissue and perfusion solution were measured using radioimmunoassay; spontaneous contraction of gastric antral circular muscles recorded using a 4-channel physiograph; and Ca^2＋-activated K^＋ currents （IK（Ca）） and spontaneous transient outward currents （STOCs） in isolated gastric antral myocytes were recorded using the whole-cell patch clamp technique. RESULTS： DNP markedly enhanced cGMP levels in gastric antral smooth muscle tissue and in the perfusion medium. DNP induced relaxation in gastric antral circular smooth muscle, which was inhibited by KT5823, a cGMP-dependent PKG inhibitor. DNP increased IK（Ca）. This effect was almost completely blocked by KT5823, and partially blocked by LY83583, an inhibitor of guanylate cyclase to change the production of cGMP. DNP also increased STOCs. The effect of DNP on STOCs was abolished in the presence of KT5823, but not affected by KT-5720, a PKA-specific inhibitor. CONCLUSION： DNP activates IK（ca） and relaxes guinea-pig gastric antral circular smooth muscle via the cGMP/PKG-dependent singling axis instead of cAMP/ PKA pathway.