中文摘要：

银杏是第四纪冰川时期孑遗的现存地球最古老的植物，为我国特有的经济树种，银杏cDNA文库的构建有助于其分子生物学研究进一步深入．以湖南银杏梅核品种优良单株成熟叶为材料，经变性裂解液分解细胞，提取总RNA，Oligotex mRNA midi kit分离纯化出mRNA，用SuperScipt TM Ⅱ RnaseH2 Reverse Transcriptase将其反转录成cDNA，与EcoRI接头连接后插入pBluescript Ⅱ SK（＋）XR载体，电转化感受态细胞DH10B中，进行菌落PCR文库质量检测．结果表明：文库总库容量为8．12×10^5克隆子，重组率为90％，文库插入片段长度约为500～2000bp．构建的银杏雌树成熟叶cDNA文库符合标准，该文库为进一步构建银杏EST文库、筛选目的基因及基因芯片制备等提供了资源基础和有效工具．

英文摘要：

Gingkgo biloba is the ancient plant living on the earth after the Ice Period of the 4th Century, and one of no-timber forests, cultivated in China, with the important economic value. Construction of a cDNA library of ginkgo is the basis of research about its molecular biology. The mature foliage, from a good tree of Hunan Meihe ginkgo cultivar, was broken up by lytic method. Total RNA was extracted, and then mRNA was extracted further, cDNA was synthesized by SuperScipt TM Ⅱ RnaseH-Reverse Transcriptase. EcoRI adaptors were ligated to the double cDNA, which was inserted into pBlueseript Ⅱ SK（＋） XR. The recombinants were electrotransformed into the E. coli DH10B. The cDNA library was tested by PCR. Results show the ginkgo library consisted of 8. 12×10^5 recombinants with the insert length of 500-2 000 bp, and the recombination efficiency was 90 %. The constructed cDNA library from the mature foliage of female ginkgo was good quality, and would be helpful to further constructing EST library, selecting target genes and preparing gene chips etc.