中文摘要：

目的探讨密蒙花总黄酮对去势雄鼠干眼症动物模型角膜及泪腺组织的保护作用与炎症反应的关系。方法150只健康1月龄Wistar雄性大鼠随机分为5组（正常组、假手术组、手术对照组、雄激素治疗组、密蒙花总黄酮治疗组,分别用A、B、C、D、E组表示）,每组30只。A组不作处理;B组只切开阴囊,不切除睾丸,作为假手术对照组;C、D、E三组实验鼠切除双侧睾丸及附睾。自造模后1周,待伤口基本愈合开始用药。A、B、C组以生理盐水灌胃,1次/天;D组用丙酸睾酮注射液大腿肌肉注射,1次/3天;E组以密蒙花提取物生理盐水混悬液灌胃,1次/天。各组实验动物分别于用药1、3、5个月后随机取10只,行Schirmer I试验（SIT）、泪膜破裂时间（break-up,BUT）及角膜荧光素染色检查并处死各组动物,取双眼泪腺、角膜组织进行光镜和透射电镜观察。结果SIT、BUT、角膜荧光素染色：A、B组这3项指标无明显变化（P0.05）;C、D、E组去势治疗1个月,角膜荧光素染色、SIT、BUT各段时间差异均无统计学意义（P0.05）;C组3个月起角膜荧光素染色阳性,且随实验时间延长而加重;5个月起泪液浸湿滤纸长度较实验前短、BUT明显低于正常对照组,且随观察时间的延长而缩短,差异有统计学意义（P0.05）;D、E组于去势3、5个月后,角膜荧光素染色点较C组减少,SIT、BUT明显高于C组,差异有统计学意义（P0.05）;D、E两组间3个月后,SIT、BUT及角膜荧光素染色差异无统计学意义（P0.05）;D、E两组间5个月后,差异有统计学意义（P0.05）。角膜和泪腺结构：A与B各组结构均无明显改变;C组自1个月起结构开始改变,且随时间延长逐渐加重;D、E两组间结构改变较C组相比明显减轻。结论密蒙花总黄酮能减轻去势雄鼠逐渐加重的泪腺分泌功能损害和角膜上皮缺失并减轻其局部炎症反应,对干眼症模型角膜和泪腺有一定的保护作用。

英文摘要：

OBJECTIVE To assess the relationships between the protective effect and tissue injury in cornea and lacrimal gland of castrated dry eye rat models by intervention of buddlija officnali flavone.METHODS One hundred and fifty healthy Wistar rats were randomly divided into 5 groups each with 30 rats：the normal group（A）,the false operation group（B）,the castrated group（C）,the therapy group treated with testosterone（D） and the therapy group with buddleja officnalis flavone（E）.Bilateral testis and epididymis of group C,D and E were resected;group B were performed scrotal incision only without removing the testicles,as sham-operated control group.Nothing was performed on group A.When the wounds were basically healing,the rats were treated with medicine 1 week after the model was established.Group A,B and C were administered saline one time a day;Group D were treated with testos-terone propionate by thigh intramuscular injection once per 3 days;Group E were treated with buddleja officnalis for one time a day.Ten experimental animals in each group were randomly chosen 1 month,3,5 months after medication respectively,which were performed Schirmer I test,tear film break-up time and corneal fluorescein staining.And then the animals were killed.The protective effect in cornea and lacrimal glands were observed by light microscopy and TEM observation.RESULTS The cornea fluorescence staining：Group A,B had not obviously changed;Group C showed positive after 3 months and aggravate as time;Group D,E the cornea fluorescence were usually dyed a bit and reduced after 3,5 months.SIT：Group C（except group C1） the wet paper was shortened,the difference was significant with before the castration（P0.05）,there was no significance with the extension difference of time（P0.05）.Group D（except group D1） compared with C,the difference was significant（P0.05）.Group E（except group D1） compared with C,the difference was significant（P0.05）.BUT：Group C（except group C1） breaks time was shortened,t