中文摘要：

目的观察中等强度静磁场对人单核细胞白血病细胞THP-1增殖和炎症因子肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6、IL-8分泌的影响。方法取对数生长期THP-1细胞,分为对照组和磁场处理组,利用60 m T、200m T、400 m T的静磁场分别作用细胞18 h、24 h,、48 h后,CCK-8法检测细胞增殖情况。另取细胞分为对照组、磁场处理组、脂多糖（LPS）活化组、LPS＋磁场组。利用60 m T、200 m T、400 m T的静磁场分别作用磁场处理组和LPS＋磁场组18 h、24 h、48 h后,酶联免疫吸附试验（ELISA）检测各组TNF-α、IL-6、IL-8的水平。结果 （1）与对照组相比,3种强度磁场处理组对THP-1细胞增殖无明显影响（P〉0.05）。（2）各组24 h时TNF-α、IL-6水平较18 h升高,IL-8则无明显变化。而48 h与24 h相比较,TNF-α出现下降,IL-6无明显变化,IL-8出现升高。3个时点LPS活化组TNF-α、IL-6、IL-8水平较对照组及磁场处理组升高,经过磁场处理后,LPS＋磁场组IL-6、IL-8、TNF-α水平均较LPS活化组下降（P〈0.05）。结论静磁场对THP-1细胞释放炎症因子有一定抑制作用,可为类风湿关节炎的治疗提供理论依据。

英文摘要：

Objective To investigate the effect of moderate static magnetic fields（SMF） on secretion of inflammato-ry factors tumor necrosis factor-α（TNF-α）, interleukin-6（IL-6） and interleukin-8（IL-8） in human monocytic leukemiccell line THP- 1. Methods THP- 1 cells at logarithmic phase were divided into control group and magnetic treatmentgroup. CCK-8 method was used to detect cell proliferation after THP-1 cells were exposed to 60 m T, 200 m T and 400 m Tstatic magnetic fields at 18, 24 and 48 h. Then THP-1 cells were divided into control group, magnetic treatment group, LPSactivation group and LPS＋SMF treatment group. When magnetic treatment group and LPS＋SMF treatment group were ex-posed to SMF at 18, 24 and 48 h, the levels of the cytokines TNF-α, IL-6 and IL-8 were determined by ELISA. Results（1）60 m T, 200 m T and 400 m T SMF had no significant effects on cell proliferation in THP-1 cells（P 〉0.05）.（2）THP-1 cellssecreted more TNF-α and IL-6 in 24 h than 18 h in every group, while IL-8 didn′t change. Compared with 24 h, the secre-tion of TNF-α decreased and IL-6 didn′t change, while IL-8 increased in 48 h. At three sampled time THP-1 cells of LPSactivation group secreted more TNF-α, IL-6, IL-8 than those of control group and magnetic treatment group. After magnetictreatment THP-1 cells of LPS＋SMF treatment group secreted less TNF-α, IL-6, IL-8 than those of LPS activation group（P 〈0.05）. Conclusion Static magnetic field may have some inhibitory effects on release of TNF-α, IL-6, IL-8 from THP-1 cells, which can provide basic data for the treatment of rheumatoid arthritis.