中文摘要：

目的探讨重组真核表达的HIV-1Nef蛋白对IL-6启动子活性的调节作用。方法将pNefF或pcI—neo与重组IL～6启动子虫荧光素酶报告质粒plL6-LUC共转染BcBL-1细胞，同时用TPA刺激同样转染的BCBL--1细胞，于转染后24h、48h、72h、96h收集细胞，用Bright—Glo虫荧光素酶检测系统进行虫荧光素酶活性检测。结果未经TPA刺激的BcBL-1细胞中，转染96h时pNefF和plL6-Luc共转染细胞中虫荧光素酶活性比pCI—neo和plL6-Luc共转染细胞增加了3．66倍。BcBL-1细胞经TPA刺激后，转染96h时，pNefF和plL6-Luc共转染细胞中虫荧光素酶下降1．72倍（P〈O．05）。结论Nef蛋白可在BCBL-1细胞内影响IL-6启动子活性。

英文摘要：

Abstract ： munodeficiency Objective virus type To investigate the role of recombinant eukaryotic expression of Nef of human im 1 （HIV--1） in activating interleukin 6 promoter. Methods Nef recombinant plas mid （pNefF） or control vector pCI--neo and luciferase reporter recombinant of IL--6 promoter （pIL6--Luc） were co--transfected to BCBL--1 cells. At the same time, other groups of BCBL--1 cell transfected with plas- mids as mentioned before were stimulated by 12--O-- tetradecanoylphorboI-- 13--acetate （TPA）. Cells were collected at 24 h, 48 h, 72 h and 96 h after transfection, respectively. Consequently, the promoter--driven lu- ciferase expression in BCBI.--1 cells was detected by Bright--Glo luciferase detect system. Results The promoter--driven luciferase expression increased by 3.66 times at 96 h after BCBL--lcells cotransfected with pNefF and pIL6--Luc without TPA when compared with ceils cotransfected with pCI--neo and pIL6--Luc. The promoter--driven luciferase expression decreased by 1.72 times at 96 h after BCBL--lcells co--transfected with pNefF and pIL6--Luc with TPA when compared with cells cotransfected with pCI--neo and plL6--Luc （P 〈 0.05） . Conclusion Expression of Nef in BCBL--1 cells contributed to activate IL--6 promoter--driv- en luciferase expression.