中文摘要：

目的探讨槟榔碱对3T3-L1前脂肪细胞增殖、分化的影响及其机制。方法采用四甲基偶氮唑盐（MTT）法和油红0染色检测不同浓度（10、30、50、100μmol／L）的槟榔碱对3T3-L1前脂肪细胞增殖和分化的影响，荧光定量PCR检测过氧化物酶体增殖物激活受体1（PPAR1）和CAAT／增强子结合蛋白α（C／EBPα）的表达。结果10～100μmol／L槟榔碱作用313-LI前脂肪细胞12h和24h能促进3T3-L1前脂肪细胞增殖，且呈-定的量效关系。到分化的第9天，与对照组相比，50μmol／L槟榔碱组胞浆中的脂滴明显减少，同时PPARγ和C／EBPαmRNA表达水平显著降低。结论槟榔碱能促进313-L1前脂肪细胞的增殖、抑制其分化，其机制可能与PPAR-γ和C／EBPα的表达降低有关。

英文摘要：

objectives To investigate the effects of arecoline on the proliferation and differentiation of 3T3-L1 pread- ipocytes and to elucidate its possible mechanism. Methods 3T3-L1 preadipocytes were cultured and treated with differ- ent concentrations of arecoline （ 10,30,50,100 μmol/L） , the proliferation and differentiation of 3T3-L1 preadipoeytes were evaluated by MTTmethods and oil red O staining ,respectively. The expressions of peroxisome proliferation activated receptorα （PPAR α） and CAAT/ enhancer binding protein （C/ EBP α） mRNAs were detected by quantitative real-time RT-PCR （qRT-PCR）. Results Treatment of arecoline at different concentrations （ 10 - 100 μ mol/L） for 12 and 24 h signifi- cantly promoted 3T3-L1 preadipocyte proliferation in a dose-depend manner. On the day 9 of differentiation, compared with the control group, lipid droplets in the cytoplasma and the PPARγ and C/EBP α mRNA expression were significantly de- creased in the 50 μmol/L arecoline group. Conclusion Arecoline promotes the proliferation and inhibits the differentia- tion of 3T3-L1 preadipocytes, which may be associated with decreased expressions of PPAR γ and C/EBP α mRNA.