中文摘要：

建立了固相萃取-高效液相色谱法同时测定植物根际促生菌发酵产物中吲哚-3-乙酸、赤霉素和玉米素3种植物激素含量的方法。固相萃取采用Agilent Bond Elut C18固相萃取柱,6 mL甲醇＋6 mL 10%甲醇活化;以5 mL 10%甲醇淋洗2次;以5mL 80%甲醇洗脱2次。采用Agilent ZORBAX Eclipse Plus C18色谱柱（250×4.6mm,5μm）分离,流动相采用体积比为2∶3的甲醇-水（含0.2%冰乙酸）二元混合溶剂,流速为1mL·min-1,柱温为30℃,进样量为20μL,检测波长为210nm。结果表明：10株供试菌株发酵产物中均能检测到3种植物激素,除菌株003NXZ4外,其余菌株均呈现出玉米素含量最高,赤霉素次之,吲哚-3-乙酸含量最低的趋势。3种植物激素在12min内分离完全,样品的加标回收率达90.3%104.3%,相对标准偏差（n=6）在1.04%2.98%之间。该方法简便、准确,可同时检测植物根际促生菌发酵产物中吲哚-3-乙酸、赤霉素和玉米素3种植物激素含量。

英文摘要：

A method of solid phase extraction-high performance liquid chromatography（SPE-HPLC）was established for the simultaneous determination of plant hormones.The purification was processed by a Agilent Bond Elut C18 solid phase extraction column.The separation was carried on Agilent ZORBAX Eclipse Plus C18column（250×4.6mm,5μm）under the detection wavelength of 210 nm.40% methanol and 60% ultrapure water（containing 0.2% acetic acid）mixture was used as the mobile phase at a flow rate of 1.0mL·min-1.The column temperature of 30℃and the injection volume of 20μL were adopted.The results shows that：3kinds of plant hormones has been found in each sample.Except 003NXZ4,the maximum content of plant hormones was trans-Zeatin.The second was gibberellin acid and the least was indole-3-acetic acid.The 3plant hormones were completely separated in 12 min.The average recovery of this method were 90.3%-104.3%,with a relative standard deviation of 1.04%-2.98%.This method is simple and accurate,and can be used for the determination of plant hormones in bacterial fermentation products of plant growth promoting rhizobacteria.