对酸浆植物的化学成分进行研究,采用反复硅胶、葡聚糖凝胶以及制备型高效液相色谱技术进行分离纯化。通过一维、二维核磁共振波谱以及质谱方法鉴定其结构。从中共分离并鉴定了14个化合物,分别为酸浆苦味素P(1),4,7-二去氢新酸浆苦味素B(2),酸浆苦味素D(3),5α-羟基-25,27-二氢-7-去氢-7-脱氧新酸浆苦味素A(4),4,7-二去氢酸浆苦味素B(5),熊果酸(6),汉黄芩素(7),布卢门醇(8),川陈皮素(9),甘草素(10),五味子醇甲(11),5-羟甲基-2-呋喃甲醛(12),5-(羟甲基)-2-(二甲氧基甲基)呋喃(13),1-O-[3—O-2-甲基-5-(2,3,4-三甲基)苯基-2,3-戊二醇1-β-木糖-(1→6),β-D-半乳糖苷(14)。其中,化合物14是一个新的化合物,化合物7-11和13为首次从该种植物中分离得到。
In the present study, in order to investigate the chemical constituents of Physal& alkekengi L. var. franchetii (Mast.) Makino, the isolation of ingredients was performed by repeated chromatography on silica gel, Sephadex LH-20 and preparative HPLC. Their structures were identified based on 1D, 2D NMR, and mass spectral analysis, A total of 14 compounds were obtained, and their structures were identified as physalin P (1), 4,7-didehydroneophysalin B (2), physalin D (3), 5α-hydroxy-25,27-dihydro-7- dehydro-7-deoxyneophysalin A (4), 4,7-didehydrophysalin B (5), ursolic acid (6), wogonin (7), blumenol A (8), nobiletin (9), liquiritigenin (10), schizandrin (11), 5-hydroxymethylfurfural (12), 5-(hydroxymethy1)-2-(dimethoxymethyl)furan (13), 1-O- [3-O-2-methyl-5-(2,3,4-trimethyl)pheny1-2,3-pentanedi]-β-D-xylopyranosyl-(1→6)-β-D-galactopyranoside (14). Among them, compound 14 is a new compound. Compounds 7-11, 13 are isolated from Physalis alkekengi L. var.franchetii (Mast.) Makino for the first time.