中文摘要：

嗜热厌氧乙醇菌遗传转化系统的缺少，制约了对该菌理论基础和应用领域的进一步研究。利用聚乙二醇（PEG6000）转化和电转化技术，国际上首次实现了嗜热厌氧乙醇菌JW200外源基因的导入。PEG转化效率很低，因此选择对电转化条件进行优化，转化效率从4±3．2个转化子／μg质粒DNA提高到50±7．4个转化子／μg质粒DNA。实验表明获得较高的转化效率的必要条件是在细胞密度为OD660 0．2时添加甘氨酸与蔗糖后，继续培养2h以及细胞在电击前的收集与洗涤保持低温。实验为利用基因工程手段改造嗜热厌氧乙醇菌，从分子水平研究胞内乙醇代谢途径奠定了基础。

英文摘要：

It is necessary to develop a genetic transformation system for Thermoanaerobacter ethanolicus JW200 to study gene function and regulation. To realize this, the gene transfer techniques for this organism were first developed. Firstly, transformation using polyethylene glycol （PEG6000） was obtained using T. ethanolicus- Escherichia coli shuttle plasmid pTE16. Secondly, electrotransformation was employed to introduce plasmid pTE16 into Thermoanaerobacter ethanolicus. Transformation efficiency of PEG method was very low, so electrotransformation procedures were optimized to enhance transformation frequency from 4 ± 3.2 to 50 ± 7.4 transformants per μg plasmid DNA. The most crucial factors conducive to achieve higher transformation efficiency included the appropriate degree of weaken cell wall and the chilling of cells during cell collection and washing. The results reported here provide the first unequivocal gene transfer method functional in T. ethanolicus that is suitable for molecular manipulations.