中文摘要：

目的：探讨消癌解毒方含药血清对人肝癌细胞SMMC-7721细胞端粒酶及凋亡相关基因mRNA表达的影响。方法：消癌解毒方含药血清作用于人肝癌SMMC-7721细胞,ELISA试剂盒检测各组端粒酶浓度,采用实时荧光定量PCR方法检测药物作用前后各组SMMC-7721细胞Bax、Bcl-2、CytC、Caspase-3 mRNA表达水平。结果：消癌解毒方含药血清作用于人肝癌SMMC-7721细胞可以降低其端粒酶浓度,且呈剂量依赖性;可使Bcl-2 mRNA表达水平下调,使Bax、CytC、Caspase-3 mRNA表达水平上调,下调Bcl-2/Bax mRNA比例,且中、高剂量组与对照组比较效果明显（P〈0.01,P〈0.05）。结论：消癌解毒方含药血清可能通过抑制端粒酶活性,调控凋亡相关基因mRNA的表达,抑制人肝癌SMMC-7721细胞生长,促使其凋亡,在肝癌治疗中发挥疗效。

英文摘要：

Objective： To study the effects of Xiao＇ai Jiedu Formula drug-containing serum on telomerase and apoptosisrelated mRNA expression in human hepatoma SMMC-7721 cells. Methods： The effects of Xiao＇ai Jiedu Formula drug-containing serum on human hepatoma SMMC-7721 cells was observed. The concentration of telomerase in each groups were measured by ELISA kits, and the expression of Bax, Bcl-2, CytC and Caspase-3 mRNA in SMMC-7721 cells were measured by Real Time PCR method before and after medication. Results： Xiao＇ai Jiedu Formula drug-containing serum could reduce the telomerase concentration of human hepatoma SMMC-7721 cells and the efficacy was dose-dependent. It was also showed that Xiao＇ai Jiedu Formula can down-regulate the Bcl-2 mRNA expression, up-regulate the expression of Bax, CytC, Caspase-3 mRNA, and decrease the Bcl-2/Bax mRNA ratio. The efficacy of Xiao＇ai Jiedu Formula was more obvious in the middle and high-dose groups than in the control group（P〈0.01, P〈0.05）. Conclusion： Xiao＇ai Jiedu Formula is able to inhibit the growth of human hepatoma SMMC-7721 cells and promote the apoptosis by suppressing telomerase activity and regulating the expression of apoptosis-related mRNA, thus playing a part in treating liver cancer.