中文摘要：

目的探讨苯并咪唑衍生物BMT-1对急性T淋巴细胞白血病Jurkat细胞增殖及凋亡的影响。方法采用CCK-8测定BMT-1对Jurkat细胞增殖的影响,采用流式细胞术检测BMT-1对Jurkat细胞的细胞周期、细胞凋亡和线粒体膜电位的影响。结果 BMT-1可显著抑制Jurkat细胞增殖,呈剂量依赖效应,GI50为1.08μmol/L。BMT-1可诱导Jurkat细胞SubG1峰显著增强、凋亡细胞比例显著增加和线粒体膜电位显著改变。结论苯并咪唑衍生物BMT-1能显著抑制Jurkat细胞增殖,并诱导细胞凋亡。

英文摘要：

Objective To explore the effect of benzimidazole derivative BMT-1 on the proliferation and apoptosis of Jurkat cells.Methods CCK-8 was used to detect the effect of BMT-1 on the proliferation of Jurkat cells,and the flow cytometer was adopted to measure the effect of BMT-1 on the cell cycle,cell apoptosis and mitochondrial membrane potential of Jurkat cells.Results BMT-1 strongly inhibited the growth of Jurkat cell with a GI50 value of 1.08 μmol/L in dose-dependent manner.It induced the enhancement of SubG1 phase of Jurkat cells,elevated the proportion of apoptosis,and changed the mitochondrial membrane potential of Jurkat cells greatly.Conclusion Benzimidazole derivative BMT-1 could significantly inhibit proliferation and induce apoptosis in Jurkat cells.