以云南省4个葡萄主产区的无核白鸡心葡萄果皮为材料,对其成分及组成进行分析。结果表明:同一品种,因其生产环境不同,基本成分及组成存在明显差异。元谋地区的葡萄皮干物质和总糖含量显著高于其他地区,可能与其高日照有关。葡萄皮中脂质的不饱和脂肪酸含量较高,葡萄皮中的单糖含量排列为葡萄糖〉阿拉伯糖〉半乳糖醛酸〉半乳糖。葡萄皮中的原花青素、多酚含量存在显著性差异,弥勒地区的含量显著高于其他地区,可能与其低海拔有一定的相关性;酚酸组成中芦丁和儿茶素含量较高,且在所有葡萄果皮中均有检出。以1,1-二苯基-2-三硝基苯肼自由基、2,2’-连氮基-双-(3-乙基苯并二氢噻唑啉-6-磺酸)二铵盐自由基清除能力和铁还原氧化能力为指标,对4个地区果皮多酚提取物的抗氧化活性进行评价,结果表明,果皮多酚具有较好的抗氧化活性,3种能力的IC50分别为12.95~14.68、8.63~11.11μg/m L和29.35~34.60μg/m L。
In this paper,the skins of Centennial Seedless from four main table grape-producing areas in Yunnan province were analyzed for chemical composition and antioxidant activities of polyphenols from grape skins were evaluated.The experimental results showed that the basic chemical components of grape skins varied with growing environment.Dry matter and total sugar contents of grape skins from Yuanmou region were significantly higher than those of grape skins from four other regions,which may be related to the higher intensity of sunlight in Yuanmou.The lipids in grape skins had a high content of unsaturated fatty acids.Arabinose,galactose,glucose,and galacturonic acid were the main monosaccharides in grape skins.Their contents in the descending order were glucosearabinosegalacturonic acidgalactose.Proanthocyanidin and polyphenol contents in grape skins from four producing areas were significantly different with significantly higher levels in Mile than in the other regions,which may be related to the lower elevations.Rutin and catechin were the man phenolic acids in grape skins.The antioxidant activity of polyphenols-rich skin extracts of grapes from four producing regions were evaluated by1,1-diphenyl-2-picrylhydrazyl(DPPH),2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid(ABTS^+·) radical scavenging capacity and reducing power(FRAP) arrays,and it was shown that the polyphenols in grape skins had high antioxidant activity with IC_(50)values of 12.95-14.68,8.63-11.11 and 29.35-34.60 μg/mL respectively.