中文摘要：

目的：观察过量氟对小鼠睾丸中骨钙素（OCN）的影响及睾酮（T）水平的变化。方法：选取24只C57black6J（C57BL／6J）雄性小鼠，随机分为两组（正常组12只，氟干预组12只），正常组饮用蒸馏水，氟干预组饮用100mg／L氟化钠溶液，自由饮用3个月，制备氟中毒模型，采用免疫组化链霉菌抗生物素蛋白一过氧化物酶连结法（sP），定位检测小鼠睾丸组织中OCN的表达采用酶联免疫吸附法（ELISA），检测血清中OCN及T水平的变化。结果：氟干预组小鼠血清中OCN水平明显高于对照组（F=11．901，P=0．008），氟干预组小鼠T分泌量明显低于对照组睾酮（F=2．313，P=0．006），免疫组化结果显示氟干预组OCN表达在细胞膜和细胞质，且阳性表达强于对照组。结论：饮用100mg／L含氟水3个月能够导致雄性小鼠的T降低，睾丸中OCN水平表达升高。

英文摘要：

Objective ： To observe the influence of excessive fluoride on the levels of osteocalein and testosterone in the testis of the male mouse. Methods： Twenty-/our C57BIM6J male mice were equally randomized into a normal control and a fnorosis model group, the former fed on distilled water while the latter on a solution of sodium fluoride （ 100 rag/I,） in distilled water, both for 12 weeks. Then, the level of osteocalcin in the testis tissue was measured with the inununohistochemical streptavidin-peroxidase （SP） method and those of osteocalcin and testosterone in the serum deternfined by ELISA. Results ： After 12 weeks of fluoride intervention, the level of serum osteoealcin was significantly higher in the fluorosis models than in the normal controls （ I68.05 ± 5. 321 vs [47.50 ± 5.731 pg/mL, F = I 1. 901, P = 0. 008）, while that of testosterone markedly lower in the former than the latter group （ 18.07 ± 1.35 ] vs [12.94 ± 3.09 ] ng/mL, F = 2. 313, P = 0. 006）. The results of inununohistochemical SP showed the expression of os- teocalcin in the tell membrane and cytoplasm of the fluorosis models, which was evidently higher than in the normal controls. Con- clusion ： Twelve-week intake of 100 mg/L fluoride solution can decrease the level of testosterone and increase the expression of osleo- ealcin in the testis of the male mouse. Natl J Androl, 2017, 23 （9） ： 782 -785