中文摘要：

从降解苯酚的好氧颗粒污泥中筛选出一株苯酚降解菌,该菌株最高可耐受2.0 g/L的苯酚。依据形态特征和内源转录间隔区（ITS）的核酸序列分析,将该菌株鉴定为热带假丝酵母菌（Candida tropicalis）,并命名为Candida tropicalis Z-04。采用中心复合设计（CCD）法对Candida tropicalis Z-04降解苯酚的条件进行合理设计,利用Design-Expert软件对试验数据进行分析,考察了各因素及其交互作用对苯酚降解率的影响,并得到苯酚最佳降解条件。结果表明,Candida tropicalis Z-04降解苯酚的最佳条件为：酵母提取物投加量0.41 g/L,苯酚初始质量浓度1.03 g/L,菌种投加量1.43%（体积分数）,温度30.04℃。在此条件下苯酚的理论预测降解率可达到最大值99.10%。

英文摘要：

A phenol-degrading strain,capable of tolerating high-strength phenol up to 2.0g/L,was isolated from phenol-degrading aerobic granules.According to its morphological and ITS gene analysis,the strain was identified as the genus Candida tropicalis,and was named Candida tropicalis Z-04.A Central Composite Design（CCD） was employed to optimize the phenol degrading conditions.The obtained experiments data were analyzed by Design-Expert software,base on which,the effect of influence factors and their interaction on phenol degradation efficiency were investigated.The results showed that the optimal degradation conditions were： yeast extract 0.41 g/L,phenol 1.03 g/L,inoculum size 1.43%（volume ratio） and temperature 30.04 ℃,and the maximum removal efficiency of phenol（99.10%） was obtained under the optimum conditions.